Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes

被引：48
|
作者：
Kong, Fanrong
Brown, Mitchell
Sabananthan, Archcna
Zeng, Xianyu
Gilbert, Gwendolyn L.
机构：
[1] Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
[2] Univ Technol Sydney, Fac Sci, Dept Cell & Mol Biol, Sydney, NSW 2007, Australia
[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW 2033, Australia
[4] Wuhan First Hosp, Wuhan 430022, Hubei Province, Peoples R China
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 05期
关键词：
D O I：
10.1128/JCM.44.5.1887-1891.2006
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-read with 1 to 4 nonvaccine serotypes, could he distinguished using serotype-specific antisera.
引用
收藏
页码：1887 / 1891
页数：5
相关论文
共 50 条
  • [41] Squamous cell carcinoma arising from an ovarian teratoma related to human papillomavirus infection: Using a PCR-based reverse-blot assay
    Liu, Li-Chun
    Huang, Rui-Lan
    Lin, Yu-Chun
    Lai, Hung-Cheng
    Su, Her-Young
    Chang, Fang-Wei
    Yu, Mu-Hsien
    TAIWANESE JOURNAL OF OBSTETRICS & GYNECOLOGY, 2011, 50 (04): : 543 - 545
  • [42] African horsesickness virus serotyping and identification of multiple co-infecting serotypes with a single genome segment 2 RT-PCR amplification and reverse line blot hybridization
    Koekemoer, JJO
    van Dijk, AA
    JOURNAL OF VIROLOGICAL METHODS, 2004, 122 (01) : 49 - 56
  • [43] The prevalence of urogenital micro-organisms detected by a multiplex PCR-reverse line blot assay in women attending three sexual health clinics in Sydney, Australia
    McKechnie, Michelle L.
    Hillman, Richard J.
    Jones, Rachel
    Lowe, Penelope C.
    Couldwel, Deborah L.
    Davies, Stephen C.
    King, Fiona
    Kong, Fanrong
    Gilbert, Gwendolyn L.
    JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (07) : 1010 - 1016
  • [44] Detection of 23S rRNA mutations associated to macrolide-resistance of Helicobacter pylori by PCR and a reverse hybridization line probe assay
    van Doorn, LJ
    Debets-Ossenkopp, YJ
    Marais, A
    van Hoek, K
    Sanna, R
    Mégraud, F
    Kusters, JG
    Quint, WGV
    GUT, 1998, 43 : A7 - A8
  • [45] Comparison of Two Widely Used Human Papillomavirus Detection and Genotyping Methods, GP5+/6+-Based PCR Followed by Reverse Line Blot Hybridization and Multiplex Type-Specific E7-Based PCR
    Clifford, Gary M.
    Vaccarella, Salvatore
    Franceschi, Silvia
    Tenet, Vanessa
    Umulisa, M. Chantal
    Tshomo, Ugyen
    Dondog, Bolormaa
    Vorsters, Alex
    Tommasino, Massimo
    Heideman, Danielle A. M.
    Snijders, Peter J. F.
    Gheit, Tarik
    JOURNAL OF CLINICAL MICROBIOLOGY, 2016, 54 (08) : 2031 - 2038
  • [46] Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 Mycobacterium species
    Xiong, Likuan
    Kong, Fanrong
    Yang, Yingzhou
    Cheng, Jinquan
    Gilbert, Gwendolyn L.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (10) : 3544 - 3550
  • [47] One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)
    Coskun-Ari, Fatma Filiz
    Guldemir, Dilek
    Durmaz, Riza
    PLOS ONE, 2012, 7 (12):
  • [48] A one-step multiplex PCR-based assay for simultaneous detection and classification of virulence factors to identify five diarrheagenic E. coli pathotypes
    Ohmura-Hoshino, Mari
    Miyaki, Yuki
    Yashima, Shigeko
    HELIYON, 2022, 8 (08)
  • [49] Simultaneously Typing Nine Serotypes of Enteroviruses Associated with Hand, Foot, and Mouth Disease by a GeXP Analyzer-Based Multiplex Reverse Transcription-PCR Assay
    Hu, Xiumei
    Zhang, Yong
    Zhou, Xiaomian
    Xu, Banglao
    Yang, Mengjie
    Wang, Miao
    Zhang, Chen
    Li, Jin
    Bai, Ruyin
    Xu, Wenbo
    Ma, Xuejun
    JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (02) : 288 - 293
  • [50] Evaluation of a PCR-based reverse hybridization system for detection of Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance.
    Glupczynski, Y
    Avesani, V
    Deprez, P
    Melange, M
    Delmee, M
    van Doorn, LJ
    GUT, 2000, 47 : A6 - A6
12345