Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes

被引：48

作者：

Kong, Fanrong

Brown, Mitchell

Sabananthan, Archcna

Zeng, Xianyu

Gilbert, Gwendolyn L.

机构：

[1] Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia

[2] Univ Technol Sydney, Fac Sci, Dept Cell & Mol Biol, Sydney, NSW 2007, Australia

[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW 2033, Australia

[4] Wuhan First Hosp, Wuhan 430022, Hubei Province, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 05期

关键词：

D O I：

10.1128/JCM.44.5.1887-1891.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-read with 1 to 4 nonvaccine serotypes, could he distinguished using serotype-specific antisera.

引用

页码：1887 / 1891

页数：5

共 50 条

[21] Identification of chromoblastomycosis agents by PCR based reverse line blot (PCR-RLB) hybridization assay
Najafzadeh, M. J.
van den Ende, A. H. G. Gerrits
Vicente, V. A.
Dolatabadi, S.
Sun, J.
de Hoog, G. S.
MICROBIAL PATHOGENESIS, 2018, 125 : 43 - 47
[22] Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
Yajuan Wang
Gaili Guo
Huixin Wang
Xuefang Yang
Fang Shao
Caiyun Yang
Wei Gao
Zhujun Shao
Jinjing Zhang
Jie Luo
Yonghong Yang
Fanrong Kong
Bingqing Zhu
BMC Pediatrics, 14
[23] Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
Wang, Yajuan
Guo, Gaili
Wang, Huixin
Yang, Xuefang
Shao, Fang
Yang, Caiyun
Gao, Wei
Shao, Zhujun
Zhang, Jinjing
Luo, Jie
Yang, Yonghong
Kong, Fanrong
Zhu, Bingqing
BMC PEDIATRICS, 2014, 14
[24] Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine
O'Halloran, DM
Cafferkey, MT
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (07) : 3487 - 3490
[25] Multiplex polymerase chain reaction-based reverse line blot hybridization assay to detect common genital pathogens
Wang, H.
Kong, F.
Wang, B.
Mckechnie, M. L.
Gilbert, G. L.
INTERNATIONAL JOURNAL OF STD & AIDS, 2010, 21 (05) : 320 - 325
[26] PCR-based assays for detection of Streptococcus pneumoniae serotypes 3, 14, 19F and 23F in respiratory specimens
Rubin, LG
Rizvi, A
JOURNAL OF MEDICAL MICROBIOLOGY, 2004, 53 (07) : 595 - 602
[27] PCR-based discrimination of emerging Streptococcus pneumoniae serotypes 22F and 33F
Gillis, Hayley D.
Demczuk, Walter H. B.
Griffith, Averil
Martin, Irene
Warhuus, Michelle
Lang, Amanda L. S.
ElSherif, May
McNeil, Shelly A.
LeBlanc, Jason J.
JOURNAL OF MICROBIOLOGICAL METHODS, 2018, 144 : 99 - 106
[28] Rapid identification of Streptococcus pneumoniae serotypes by cpsB gene-based sequetyping combined with multiplex PCR
Zhou, Meng-Lan
Wang, Zi-Ran
Li, Yan-Bing
Kudinha, Timothy
Wang, Jian
Wang, Yao
Xiao, Meng
Xu, Ying-Chun
Liu, Zheng-Yin
Hsueh, Po-Ren
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, 2022, 55 (05) : 870 - 879
[29] Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
Ziane, Hanifa
Manageiro, Vera
Ferreira, Eugenia
Bektache, Soumia
Tazir, Mohamed
Canica, Manuela
JOURNAL OF MICROBIOLOGICAL METHODS, 2015, 119 : 243 - 246
[30] Use of PCR and reverse line blot hybridization assay for rapid simultaneous detection and serovar identification of Chlamydia trachomatis
Xiong, LK
Kong, FR
Zhou, H
Gilbert, GL
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (04) : 1413 - 1418

← 1 2 3 4 5 →