Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes

被引：48

作者：

Kong, Fanrong

Brown, Mitchell

Sabananthan, Archcna

Zeng, Xianyu

Gilbert, Gwendolyn L.

机构：

[1] Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia

[2] Univ Technol Sydney, Fac Sci, Dept Cell & Mol Biol, Sydney, NSW 2007, Australia

[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW 2033, Australia

[4] Wuhan First Hosp, Wuhan 430022, Hubei Province, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 05期

关键词：

D O I：

10.1128/JCM.44.5.1887-1891.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-read with 1 to 4 nonvaccine serotypes, could he distinguished using serotype-specific antisera.

引用

页码：1887 / 1891

页数：5

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