Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes

被引：48
|
作者：
Kong, Fanrong
Brown, Mitchell
Sabananthan, Archcna
Zeng, Xianyu
Gilbert, Gwendolyn L.
机构：
[1] Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
[2] Univ Technol Sydney, Fac Sci, Dept Cell & Mol Biol, Sydney, NSW 2007, Australia
[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW 2033, Australia
[4] Wuhan First Hosp, Wuhan 430022, Hubei Province, Peoples R China
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 05期
关键词：
D O I：
10.1128/JCM.44.5.1887-1891.2006
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-read with 1 to 4 nonvaccine serotypes, could he distinguished using serotype-specific antisera.
引用
收藏
页码：1887 / 1891
页数：5
相关论文
共 50 条
  • [1] Identification of less-common Streptococcus pneumoniae serotypes by a multiplex PCR-based reverse line blot hybridization assay
    Zhou, Fei
    Kong, Fanrong
    Tong, Zhongsheng
    Gilbert, Gwendolyn L.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (10) : 3411 - 3415
  • [2] Molecular serotype identification of Streptococcus agalactiae of bovine origin by multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay
    Zhao, Zuotao
    Kong, Fanrong
    Martinez, Gabriela
    Zeng, Xianyu
    Gottschalk, Marcelo
    Gilbert, Gwendolyn L.
    FEMS MICROBIOLOGY LETTERS, 2006, 263 (02) : 236 - 239
  • [3] Evaluation of a multiplex PCR-based reverse line blot-hybridization assay for identification of serotype and surface protein antigens of Streptococcus agalactiae
    Zeng, Xianyu
    Kong, Fanrong
    Morgan, Julie
    Gilbert, Gwendolyn L.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (10) : 3822 - 3825
  • [4] Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB) - a practical epidemiological and diagnostic tool
    Kong, Fanrong
    Gilbert, Gwendolyn L.
    NATURE PROTOCOLS, 2006, 1 (06) : 2668 - 2680
  • [5] Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB)—a practical epidemiological and diagnostic tool
    Fanrong Kong
    Gwendolyn L Gilbert
    Nature Protocols, 2006, 1 : 2668 - 2680
  • [6] Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
    O'Sullivan, Matthew V. N.
    Zhou, Fei
    Sintchenko, Vitali
    Kong, Fanrong
    Gilbert, Gwendolyn L.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2011, (54):
  • [7] A multiplex PCR-based reverse line blot hybridization (mPCR/RLB) assay for detection of bacterial respiratory pathogens in children with pneumonia
    Wang, Yajuan
    Kong, Fanrong
    Yang, Yonghong
    Gilbert, Gwendolyn L.
    PEDIATRIC PULMONOLOGY, 2008, 43 (02) : 150 - 159
  • [8] Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR-Based Reverse Line Blot Assay
    McKechnie, Michelle L.
    Hillman, Richard
    Couldwell, Deborah
    Kong, Fanrong
    Freedman, Eleanor
    Wang, Hui
    Gilbert, Gwendolyn L.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (06) : 1871 - 1877
  • [9] Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization
    Kong, F
    Ma, L
    Gilbert, GL
    JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (12) : 1133 - 1138
  • [10] Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription-PCR-Based Reverse Line Blot Hybridization Assay
    Zhou, Fei
    Kong, Fanrong
    McPhie, Kenneth
    Ratnamohan, Mala
    Donovan, Linda
    Zeng, Frank
    Gilbert, Gwendolyn L.
    Dwyer, Dominic E.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (09) : 2737 - 2743
12345