Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays

被引：44
|
作者：
Freisleben, A [1 ]
Schieberle, P [1 ]
Rychlik, M [1 ]
机构：
[1] Tech Univ Munich, Inst Lebensmittelchem, D-85748 Garching, Germany
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2002年 / 50卷 / 17期
关键词：
electrospray mass spectrometry; folates; labeled folic acid; stable isotope dilution assay;
D O I：
10.1021/jf025571k
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
[H-2(4)]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [H-2(4)]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.
引用
收藏
页码：4760 / 4768
页数：9
相关论文
共 50 条
  • [21] Stable isotope labeled target compounds: Preparation and use as internal standards in quantitative mass spectrometry
    Dept. Biochem. Anal./Mass Spectrom., Univ. Childrens Hospital, Auenbruggerplatz 30, A-8036 Graz, Austria
    Curr. Org. Chem., 2 (131-144):
  • [22] Stable isotope dilution assays as tools for assessing folate bioavailability
    Rychlik, Michael
    Moench, Sabine
    Netzel, Michael
    Netzel, Gabriele
    Oehrvik, Veronica
    Buettner, Barbara
    Lundin, Eva
    Witthoeft, Cornelia M.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2012, 243
  • [23] Mycotoxins in cereal products: Quantitation by stable isotope dilution assays
    Rychlik, M
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 230 : U69 - U69
  • [24] Development of stable isotope dilution assays for ochratoxin A in blood samples
    Korn, Markus
    Frank, Oliver
    Hofmann, Thomas
    Rychlik, Michael
    ANALYTICAL BIOCHEMISTRY, 2011, 419 (02) : 88 - 94
  • [25] Stable isotope labeled target compounds: Preparation and use as internal standards in quantitative mass spectrometry
    Leis, HJ
    Fauler, G
    Windischhofer, W
    CURRENT ORGANIC CHEMISTRY, 1998, 2 (02) : 131 - 144
  • [26] Scalable synthesis of the aroma compounds d6-β-ionone and d6-β-cyclocitral for use as internal standards in stable isotope dilution assays
    Mosaferi, Shabnam
    Jelley, Rebecca E.
    Fedrizzi, Bruno
    Barker, David
    TETRAHEDRON LETTERS, 2020, 61 (51)
  • [27] Synthesis of deuterium-labelled fluorobenzoic acids to be used as internal standards in isotope dilution mass spectrometry
    Mueller, Karsten
    Seubert, Andreas
    ISOTOPES IN ENVIRONMENTAL AND HEALTH STUDIES, 2014, 50 (01) : 88 - 93
  • [28] Quantitative metabolomics for dynamic metabolic engineering using stable isotope labeled internal standards mixture (SILIS)
    Soma, Yuki
    Takahashi, Masatomo
    Fujiwara, Yuri
    Tomiyasu, Noriyuki
    Goto, Maiko
    Hanai, Taizo
    Izumi, Yoshihiro
    Bamba, Takeshi
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2022, 133 (01) : 46 - 55
  • [29] Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics
    Narumi, Ryohei
    Masuda, Keiko
    Tomonaga, Takeshi
    Adachi, Jun
    Ueda, Hiroki R.
    Shimizu, Yoshihiro
    SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2018, 3 (02) : 97 - 104
  • [30] Biosynthesis of 15N-labeled cylindrospermopsin and its application as internal standard in stable isotope dilution analysis
    Katrin Kittler
    Holger Hoffmann
    Franziska Lindemann
    Matthias Koch
    Sascha Rohn
    Ronald Maul
    Analytical and Bioanalytical Chemistry, 2014, 406 : 5765 - 5774
12345