Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays

被引：44
|
作者：
Freisleben, A [1 ]
Schieberle, P [1 ]
Rychlik, M [1 ]
机构：
[1] Tech Univ Munich, Inst Lebensmittelchem, D-85748 Garching, Germany
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2002年 / 50卷 / 17期
关键词：
electrospray mass spectrometry; folates; labeled folic acid; stable isotope dilution assay;
D O I：
10.1021/jf025571k
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
[H-2(4)]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [H-2(4)]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.
引用
收藏
页码：4760 / 4768
页数：9
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