DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

被引：0
|
作者：
Weiner, Anna [1 ]
Golebiowska, Agata [1 ]
Paprocka, Ilona [1 ]
Kwiatek, Krzysztof [1 ]
机构：
[1] Natl Inst Vet Res, Dept Hyg Anim Feedingstuffs, PL-24100 Pulawy, Poland
来源：
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2013年 / 57卷 / 04期
关键词：
bovine DNA; feeds; meat and bone meal; processed animal proteins; PCR; POLYMERASE-CHAIN-REACTION; PROCESSED ANIMAL PROTEINS; SPECIES-SPECIFIC PCR; SPONGIFORM ENCEPHALOPATHY; MITOCHONDRIAL-DNA; RUMINANT FEEDS; TISSUES; PRODUCTS; ASSAY; FEEDINGSTUFFS;
D O I：
10.2478/bvip-2013-0095
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of this paper was to present the results of comparative evaluation of the usefulness of PCR method for the detection and identification of bovine DNA in feeds. In the validation study, the limit of detection for PCR was determined as 0.05% for bovine meat and bone meal (MBM). Among 132 feed samples, bovine DNA was detected in eight (6.06%) samples. In the next stage of the study, sediment and flotate from the investigated samples were examined with PCR. Out of 132 sediment and notate samples, bovine DNA was detected in eight (6.06%) and nine (6.82%) samples, respectively. On the basis of the results obtained with the use of the PCR, it is possible to state that the molecular biology methods can, at present, be used as supplementaiy tools for detection and identification of bovine MBM.
引用
收藏
页码：553 / 557
页数：5
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