Production of monoclonal antibody for the detection of meat and bone meal in animal feed

被引：14

作者：

Kim, SH ^{[1
]}

Huang, TS

Seymour, TA

Wei, CI

Kempf, SC

Bridgman, CR

Clemens, RA

An, HJ

机构：

[1] Oklahoma State Univ, Col Human Environm Sci, Dept Nutr Sci, Stillwater, OK 74078 USA

[2] Auburn Univ, Dept Nutr & Food Sci, Auburn, AL 36849 USA

[3] Auburn Univ, Hybridoma Facil, Auburn, AL 36849 USA

[4] Univ So Calif, Sch Pharm, Los Angeles, CA 90089 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 25期

关键词：

meat and bone meal; animal feed; monoclonal antibody; ELISA; immunoassay;

D O I：

10.1021/jf048789a

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

For the detection of prohibited meat and bone meal (MBM) in animal feed, monoclonal antibodies (MAbs) were raised against heat-stable h-caldesmon purified from bovine intestinal smooth muscle. The obtained hybridoma cells were screened against extracts of the bovine MBM and heat-treated smooth muscle, and MAb 5E12 was identified as having the best performance. Antibody 5E12 did not react with animal feed, milk product, plant proteins, and other ingredients used for commercial animal feed except for the gelatin. This antibody diluted to 100-fold was able to detect MBM mixed in animal feed at 0.05% in an ELISA, and it showed strong affinity toward bovine smooth muscle autoclaved at 130 degreesC. Therefore, this antibody can be used in the ELISA system for field testing of the presence of MBM in animal feed.

引用

页码：7580 / 7585

页数：6

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