DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

被引：0
|
作者：
Weiner, Anna [1 ]
Golebiowska, Agata [1 ]
Paprocka, Ilona [1 ]
Kwiatek, Krzysztof [1 ]
机构：
[1] Natl Inst Vet Res, Dept Hyg Anim Feedingstuffs, PL-24100 Pulawy, Poland
来源：
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2013年 / 57卷 / 04期
关键词：
bovine DNA; feeds; meat and bone meal; processed animal proteins; PCR; POLYMERASE-CHAIN-REACTION; PROCESSED ANIMAL PROTEINS; SPECIES-SPECIFIC PCR; SPONGIFORM ENCEPHALOPATHY; MITOCHONDRIAL-DNA; RUMINANT FEEDS; TISSUES; PRODUCTS; ASSAY; FEEDINGSTUFFS;
D O I：
10.2478/bvip-2013-0095
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of this paper was to present the results of comparative evaluation of the usefulness of PCR method for the detection and identification of bovine DNA in feeds. In the validation study, the limit of detection for PCR was determined as 0.05% for bovine meat and bone meal (MBM). Among 132 feed samples, bovine DNA was detected in eight (6.06%) samples. In the next stage of the study, sediment and flotate from the investigated samples were examined with PCR. Out of 132 sediment and notate samples, bovine DNA was detected in eight (6.06%) and nine (6.82%) samples, respectively. On the basis of the results obtained with the use of the PCR, it is possible to state that the molecular biology methods can, at present, be used as supplementaiy tools for detection and identification of bovine MBM.
引用
收藏
页码：553 / 557
页数：5
相关论文
共 50 条
  • [31] Advancing meat safety diverse approaches for bovine tuberculosis detection and control
    Elbarbary, Nady Khairy
    Al-Qaaneh, Ayman M.
    Bekhit, Mounir M.
    Fotouh, Ahmed
    Madkour, Bahaa S.
    Malak, Nermeen M. L.
    Hadad, Ghada
    Maher, Zainab M.
    Salem, Mohamed M.
    Abdelhaseib, Maha
    ITALIAN JOURNAL OF FOOD SCIENCE, 2024, 36 (04) : 240 - 254
  • [32] Traceability of bovine meat and bone meal in eggs from laying hens fed with alternative ingredients
    Denadai, Juliana Celia
    Ducatti, Carlos
    Sartori, Jose Roberto
    Pezzato, Antonio Celso
    Mori, Cleusa
    Gottmann, Rosana
    Okino Mituo, Mariela Akie
    PESQUISA AGROPECUARIA BRASILEIRA, 2009, 44 (01) : 1 - 7
  • [33] The Use of Carbon and Nitrogen Stable Isotopes for the Detection of Poultry Offal Meal in Meat-Type Quail Feeds
    Sernagiotto, E. R.
    Ducatti, C.
    Sartori, J. R.
    Stradiotti, A. C.
    Maruno, M. K.
    Araujo, P. C.
    Carvalho, F. B.
    Pezzato, A. C.
    BRAZILIAN JOURNAL OF POULTRY SCIENCE, 2013, 15 (01) : 65 - 70
  • [34] DNA BASED GENDER IDENTIFICATION OF MEAT SAMPLES
    Iqbal, M.
    Shehzad, W.
    Imran, M.
    Zahoor, M. Y.
    Khan, W. A.
    Rashid, M., I
    Ashraf, K.
    Rehman, H.
    Saleem, M. S.
    Nadeem, A.
    JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS, 2020, 30 (06): : 1374 - 1379
  • [35] Detection of bovine mitochondrial DNA in ruminant feeds: A molecular approach to test for the presence of bovine-derived materials
    Tartaglia, M
    Saulle, E
    Pestalozza, S
    Morelli, L
    Antonucci, G
    Battaglia, PA
    JOURNAL OF FOOD PROTECTION, 1998, 61 (05) : 513 - 518
  • [36] A Markov random field based approach to the identification of meat and bone meal in feed by near-infrared spectroscopic imaging
    Xunpeng Jiang
    Zengling Yang
    Lujia Han
    Analytical and Bioanalytical Chemistry, 2014, 406 : 4705 - 4714
  • [37] A Markov random field based approach to the identification of meat and bone meal in feed by near-infrared spectroscopic imaging
    Jiang, Xunpeng
    Yang, Zengling
    Han, Lujia
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (19) : 4705 - 4714
  • [38] Inactivation of BSE Prion and Recycling of Bovine Meat and Bone Meal by Sub-Critical Water Treatment
    Tsutsumi, Yuji
    Bang, Hyun-Jung
    Takata, Eri
    Fukuda, Atsuko
    Murayama, Yuich
    Yoshioka, Miyako
    Noguchi, Takashi
    PRION, 2011, 5 : 110 - 111
  • [39] A quantitative assessment of the risk of exposure to bovine spongiform encephalopathy meal via meat-and-bone in Japan
    Yamamoto, Takehisa
    Tsutsui, Toshiyuki
    Nonaka, Takashi
    Kobayashi, Sota
    Nishiguchi, Akiko
    Yamane, Itsuro
    PREVENTIVE VETERINARY MEDICINE, 2006, 75 (3-4) : 221 - 238
  • [40] Markers mining for species discrimination based on component characteristics of meat and bone meal
    Gao B.
    Wang M.
    Yao Y.
    Gao F.
    Han L.
    Liu X.
    Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering, 2020, 36 (18): : 275 - 282
12345