Immunoprecipitation to Examine Protein Complexes

被引：1
|
作者：
Pazour, Gregory J. [1 ]
机构：
[1] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA
来源：
CILIA: STRUCTURE AND MOTILITY | 2009年 / 91卷
关键词：
MICROTUBULE;
D O I：
10.1016/S0091-679X(08)91008-6
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Immunoprecipitation approaches are widely used in the field of cell biology. This method is ideally suited to probe interactions between proteins that make up highly complex organelles like eukaryotic cilia and flagella. In this chapter, I discuss general methods for carrying out and analyzing immunoprecipitation experiments and discuss the controls that should be included in all valid immunoprecipitation experiments.
引用
收藏
页码：135 / 142
页数：8
相关论文
共 50 条
  • [41] STAR PHENOMENON, A 3 COMPONENT IMMUNOPRECIPITATION INVOLVING PROTEIN-A
    KRONVALL, C
    WILLIAMS, RC
    IMMUNOCHEMISTRY, 1971, 8 (06): : 577 - &
  • [42] Enhanced Ribonucleoprotein Immunoprecipitation (RIP) Technique for the Identification of mRNA Species in Ribonucleoprotein Complexes
    Fakhraldeen, Saja A.
    Berry, Scott M.
    Beebe, David J.
    Alexander, Caroline M.
    BIO-PROTOCOL, 2022, 12 (19):
  • [43] Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) for analysis of chromatin complexes
    Mohammed, Hisham
    Taylor, Christopher
    Brown, Gordon D.
    Papachristou, Evaggelia K.
    Carroll, Jason S.
    D'Santos, Clive S.
    NATURE PROTOCOLS, 2016, 11 (02) : 316 - 326
  • [44] Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) for analysis of chromatin complexes
    Hisham Mohammed
    Christopher Taylor
    Gordon D Brown
    Evaggelia K Papachristou
    Jason S Carroll
    Clive S D'Santos
    Nature Protocols, 2016, 11 : 316 - 326
  • [45] Immunoprecipitation of Native Botulinum Neurotoxin Complexes from Clostridium botulinum Subtype A Strains
    Lin, Guangyun
    Tepp, William H.
    Bradshaw, Marite
    Fredrick, Chase M.
    Johnson, Eric A.
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2015, 81 (02) : 481 - 491
  • [46] TAENIA-TAENIAEFORMIS - IMMUNOPRECIPITATION ANALYSIS OF THE PROTEIN ANTIGENS OF ONCOSPHERES AND LARVAE
    BOWTELL, DDL
    MITCHELL, GF
    ANDERS, RF
    LIGHTOWLERS, MW
    RICKARD, MD
    EXPERIMENTAL PARASITOLOGY, 1983, 56 (03) : 416 - 427
  • [47] The Use of Specific Immunoprecipitation to Monitor Phosphorylation of a Microtubule-Associated Protein
    Sheterline, Peter
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1978, 6 : 231 - 233
  • [48] A SIMPLIFIED FORMALDEHYDE FIXATION AND IMMUNOPRECIPITATION TECHNIQUE FOR STUDYING PROTEIN DNA INTERACTIONS
    DEDON, PC
    SOULTS, JA
    ALLIS, CD
    GOROVSKY, MA
    ANALYTICAL BIOCHEMISTRY, 1991, 197 (01) : 83 - 90
  • [49] Evaluation of His-tag and immunoprecipitation procedures for recombinant protein purification
    Lin, Rui
    Cao, Heping
    FASEB JOURNAL, 2009, 23
  • [50] Immunoprecipitation of serum albumin with protein A-Sepharose - A biochemistry laboratory experiment
    Bohinski, RC
    JOURNAL OF CHEMICAL EDUCATION, 2000, 77 (11) : 1460 - 1462
12345