Immunoprecipitation to Examine Protein Complexes

被引:1
|
作者
Pazour, Gregory J. [1 ]
机构
[1] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA
来源
关键词
MICROTUBULE;
D O I
10.1016/S0091-679X(08)91008-6
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Immunoprecipitation approaches are widely used in the field of cell biology. This method is ideally suited to probe interactions between proteins that make up highly complex organelles like eukaryotic cilia and flagella. In this chapter, I discuss general methods for carrying out and analyzing immunoprecipitation experiments and discuss the controls that should be included in all valid immunoprecipitation experiments.
引用
收藏
页码:135 / 142
页数:8
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