Immunoprecipitation to Examine Protein Complexes

被引：1
|
作者：
Pazour, Gregory J. [1 ]
机构：
[1] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA
来源：
CILIA: STRUCTURE AND MOTILITY | 2009年 / 91卷
关键词：
MICROTUBULE;
D O I：
10.1016/S0091-679X(08)91008-6
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Immunoprecipitation approaches are widely used in the field of cell biology. This method is ideally suited to probe interactions between proteins that make up highly complex organelles like eukaryotic cilia and flagella. In this chapter, I discuss general methods for carrying out and analyzing immunoprecipitation experiments and discuss the controls that should be included in all valid immunoprecipitation experiments.
引用
收藏
页码：135 / 142
页数：8
相关论文
共 50 条
  • [31] PROTEIN TURNOVER STUDIES USING INVIVO LABELING AND IMMUNOPRECIPITATION
    JOHNSON, AM
    MCMILLAN, CW
    CLINICA CHIMICA ACTA, 1975, 62 (02) : 363 - 369
  • [32] Dissecting interactions between interleukin-1alpha and histone acetyltransferase complexes by in vivo protein-tagging and immunoprecipitation techniques
    Vicenova, Blanka
    Buryskova, Miroslava
    Burysek, Ladislav
    Novak, Josef
    Pospisek, Martin
    CYTOKINE, 2009, 48 (1-2) : 73 - 73
  • [33] Immunoprecipitation for rapid detection of macroprolactin in the form of prolactin-immunoglobulin complexes
    Schiettecatte, J
    Van Opdenbosch, A
    Anckaert, E
    De Schepper, J
    Poppe, K
    Velkeniers, B
    Smitz, J
    CLINICAL CHEMISTRY, 2005, 51 (09) : 1746 - 1748
  • [34] Protein interaction screening by quantitative immunoprecipitation combined with knockdown (QUICK)
    Matthias Selbach
    Matthias Mann
    Nature Methods, 2006, 3 : 981 - 983
  • [35] Chromatin immunoprecipitation and microarray-based analysis of protein location
    Tong Ihn Lee
    Sarah E Johnstone
    Richard A Young
    Nature Protocols, 2006, 1 : 729 - 748
  • [36] IMMUNOPRECIPITATION AND PROTEIN ASSAYS - INFLUENCE OF BLANK MEASURING ON RESULTS TRANSFERABILITY
    DAUNIZEAU, A
    ONRAED, B
    MOINS, MP
    BERNARD, M
    FOGLIETTI, MJ
    DOSBAA, I
    AITBACHIR, N
    CLINICAL CHEMISTRY, 1995, 41 (06) : S148 - S149
  • [37] Microfluidic Immunoprecipitation for Post-Translational Modified Protein Purification
    XIA Hui1
    Chinese Journal of Biomedical Engineering, 2012, 21 (02) : 80 - 86
  • [38] Chromatin immunoprecipitation and microarray-based analysis of protein location
    Lee, Tong Ihn
    Johnstone, Sarah E.
    Young, Richard A.
    NATURE PROTOCOLS, 2006, 1 (02) : 729 - 748
  • [39] DSP-crosslinking and Immunoprecipitation to Isolate Weak Protein Complex
    Akaki, Kotaro
    Mino, Takashi
    Takeuchi, Osamu
    BIO-PROTOCOL, 2022, 12 (15):
  • [40] Protein interaction screening by quantitative immunoprecipitation combined with knockdown (QUICK)
    Selbach, Matthias
    Mann, Matthias
    NATURE METHODS, 2006, 3 (12) : 981 - 983
12345