Molecular cloning of a gene encoding a 20S proteasome β subunit from Plasmodium falciparum

被引：9
|
作者：
Li, GD
Li, JL
Mugthin, M
Ward, SA
机构：
[1] Univ Liverpool, Dept Pharmacol & Therapeut, Liverpool L69 3BX, Merseyside, England
[2] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England
来源：
INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2000年 / 30卷 / 06期
基金：
英国惠康基金;
关键词：
beta-subunit; multicatalytic protease complex; Plasmodium falciparum; proteasome;
D O I：
10.1016/S0020-7519(00)00046-1
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome P-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a I56-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding. (C) 2000 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：729 / 733
页数：5
相关论文
共 50 条
  • [31] Metal resistance in yeast mediated by the expression of a maize 20S proteasome α subunit
    Forzani, C
    Lobréaux, S
    Mari, S
    Briat, JF
    Lebrun, M
    GENE, 2002, 293 (1-2) : 199 - 204
  • [32] SUBUNIT OF THE 20S PROTEASOME (MULTICATALYTIC PROTEINASE) ENCODED BY THE MAJOR HISTOCOMPATIBILITY COMPLEX
    ORTIZNAVARRETE, V
    SEELIG, A
    GERNOLD, M
    FRENTZEL, S
    KLOETZEL, PM
    HAMMERLING, GJ
    NATURE, 1991, 353 (6345) : 662 - 664
  • [33] A third interferon-gamma-induced subunit exchange in the 20S proteasome
    Groettrup, M
    Kraft, R
    Kostka, S
    Standera, S
    Stohwasser, R
    Kloetzel, PM
    EUROPEAN JOURNAL OF IMMUNOLOGY, 1996, 26 (04) : 863 - 869
  • [34] Identification of the goldfish 20S proteasome β6 subunit bound to nuclear matrix
    Tokumoto, M
    Yamaguchi, A
    Nagahama, Y
    Tokumoto, T
    FEBS LETTERS, 2000, 472 (01) : 62 - 66
  • [35] Characterization of the 20S proteasome from the actinomycete Frankia
    Pouch, MN
    Cournoyer, B
    Baumeister, W
    MOLECULAR MICROBIOLOGY, 2000, 35 (02) : 368 - 377
  • [36] CLONING AND SEQUENCING OF THE GENE ENCODING THE LARGE (ALPHA-) SUBUNIT OF THE PROTEASOME FROM THERMOPLASMA-ACIDOPHILUM
    ZWICKL, P
    LOTTSPEICH, F
    DAHLMANN, B
    BAUMEISTER, W
    FEBS LETTERS, 1991, 278 (02) : 217 - 221
  • [37] Characterization and cloning of the gene encoding the vacuolar membrane protein EXP-2 from Plasmodium falciparum
    Fischer, K
    Marti, T
    Rick, B
    Johnson, D
    Benting, J
    Baumeister, S
    Helmbrecht, C
    Lanzer, M
    Lingelbach, K
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1998, 92 (01) : 47 - 57
  • [38] Molecular characterization of a Leishmania donovanii cDNA clone with similarity to human 20S proteasome a-type subunit
    Christensen, CBV
    Jorgensen, L
    Jensen, ATR
    Gasim, S
    Chen, M
    Kharazmi, A
    Theander, TG
    Andresen, K
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2000, 1500 (01): : 77 - 87
  • [39] Cereblon inhibits proteasome activity by binding to the 20S core proteasome subunit beta type 4
    Lee, Kwang Min
    Lee, Jongwon
    Park, Chul-Seung
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012, 427 (03) : 618 - 622
  • [40] Molecular characterization of a Plasmodium falciparum gene encoding the mitochondrial phosphate carrier
    BhaduriMcIntosh, S
    Vaidya, AB
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 78 (1-2) : 297 - 301
12345