Molecular cloning of a gene encoding a 20S proteasome β subunit from Plasmodium falciparum

被引：9
|
作者：
Li, GD
Li, JL
Mugthin, M
Ward, SA
机构：
[1] Univ Liverpool, Dept Pharmacol & Therapeut, Liverpool L69 3BX, Merseyside, England
[2] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England
来源：
INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2000年 / 30卷 / 06期
基金：
英国惠康基金;
关键词：
beta-subunit; multicatalytic protease complex; Plasmodium falciparum; proteasome;
D O I：
10.1016/S0020-7519(00)00046-1
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome P-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a I56-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding. (C) 2000 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：729 / 733
页数：5
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