Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, chicken and turkey

被引：75
|
作者：
Koeppel, Rene [1 ]
Ruf, Jueg [2 ]
Zimmerli, Franziska [1 ]
Breitenmoser, Alda [1 ]
机构：
[1] Offcial Food Control Author Canton Zurich, Zurich, Switzerland
[2] Offcial Food Control Author Canton Thurgau, Zurich, Switzerland
来源：
EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2008年 / 227卷 / 04期
关键词：
multiplex; real-time quantitative PCR; beef; pork; chicken; turkey;
D O I：
10.1007/s00217-008-0837-7
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Many meat products are composed of two or more meat species. To determine the proportion of these meat fractions, a quantitative multiplex PCR was developed for the quantification of beef, pork, chicken and turkey. This system proved its applicability, precision and accuracy in examining different meat products from the market. Thus it allows the efficient control of composed meat products in official food control and production control laboratories.
引用
收藏
页码：1199 / 1203
页数：5
相关论文
共 50 条
  • [31] Detection and quantification of Campylobacter jejuni and Campylobacter coli using real-time multiplex PCR
    Toplak, N.
    Kovac, M.
    Piskernik, S.
    Mozina, S. Smole
    Jersek, B.
    JOURNAL OF APPLIED MICROBIOLOGY, 2012, 112 (04) : 752 - 764
  • [32] Kinetic models and quantification algorithms for multiplex real-time PCR
    Keener, J
    Chagovetz, A
    FASEB JOURNAL, 2004, 18 (08): : C152 - C152
  • [33] Detection of Chicken and Turkey Meat in Meat Mixtures by Using Real-Time PCR Assays
    Kesmen, Zulal
    Yetiman, Ahmet E.
    Sahin, Fikrettin
    Yetim, Hasan
    JOURNAL OF FOOD SCIENCE, 2012, 77 (02) : C167 - C173
  • [34] Multiplex real-time PCR detection of Vibrio cholerae
    Gubala, Aneta J.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 65 (02) : 278 - 293
  • [35] Multiplex real-time PCR for dermatophytes detection and identification
    Arabatzis, M.
    van Coppenraet, L. Bruijnesteijn
    Kuijper, E.
    de Hoog, S.
    Lavrijsen, A.
    Templeton, K.
    van der Raaij-Helmer, E.
    Velegraki, A.
    Summerbell, R.
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2006, 126 : 128 - 128
  • [36] Development of a real-time PCR protocol for the detection of chicken DNA in meat products
    Abitayeva, Gulyaim
    Abeev, Arman
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2024, 54 (08): : 1068 - 1078
  • [37] Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples
    Edel O'Regan
    Evonne McCabe
    Catherine Burgess
    Sheila McGuinness
    Thomas Barry
    Geraldine Duffy
    Paul Whyte
    Séamus Fanning
    BMC Microbiology, 8
  • [38] Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples
    O'Regan, Edel
    McCabe, Evonne
    Burgess, Catherine
    McGuinness, Sheila
    Barry, Thomas
    Duffy, Geraldine
    Whyte, Paul
    Fanning, Seamus
    BMC MICROBIOLOGY, 2008, 8 (1)
  • [39] Quantification of beef, pork, chicken and turkey proportions in sausages: use of matrix-adapted standards and comparison of single versus multiplex PCR in an interlaboratory trial
    Eugster, Albert
    Ruf, Juerg
    Rentsch, Juerg
    Koeppel, Rene
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2009, 230 (01) : 55 - 61
  • [40] Quantification of beef, pork, chicken and turkey proportions in sausages: use of matrix-adapted standards and comparison of single versus multiplex PCR in an interlaboratory trial
    Albert Eugster
    Jürg Ruf
    Jürg Rentsch
    René Köppel
    European Food Research and Technology, 2009, 230 : 55 - 61
12345