Simple microdilution test for detection of metallo-β-lactamase production in Pseudomonas aeruginosa

被引:70
|
作者
Migliavacca, R
Docquier, JD
Mugnaioli, C
Amicosante, G
Daturi, R
Lee, KW
Rossolini, GM
Pagani, L
机构
[1] Univ Pavia, Sez Nucribuik, Dipartimento Sci Morfol Eidol & Clin, I-27100 Pavia, Italy
[2] IRCCS S Matteo, Serv Anal Microbiol, I-27100 Pavia, Italy
[3] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy
[4] Univ Aquila, Dipartimento Sci & Tecnol BIomed, I-67100 Laquila, Italy
[5] Yonsei Univ, Coll Med, Dept Clin Pathol, Seoul 120749, South Korea
[6] Yonsei Univ, Coll Med, Res Inst Bacterial Resistance, Seoul 120749, South Korea
关键词
D O I
10.1128/JCM.40.11.4388-4390.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18VIM- and 4 IMP-type metallo-beta-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.
引用
收藏
页码:4388 / 4390
页数:3
相关论文
共 50 条
  • [41] High Prevalence of Metallo-β-lactamase-Producing Pseudomonas aeruginosa from Saudi Arabia
    Al-Agamy, M. H. M.
    Shibl, A. M.
    Tawfik, A. F.
    Radwan, H. H.
    JOURNAL OF CHEMOTHERAPY, 2009, 21 (04) : 461 - 462
  • [42] Alcaligenes faecalis metallo-β-lactamase in extensively drug-resistant Pseudomonas aeruginosa isolates
    Li, Yue
    Zhu, Yiwei
    Zhou, Wanqing
    Chen, Zhongju
    Moran, Robert A.
    Ke, Huanhuan
    Feng, Yu
    van Schaik, Willem
    Shen, Han
    Ji, Jingshu
    Ruan, Zhi
    Hua, Xiaoting
    Yu, Yunsong
    CLINICAL MICROBIOLOGY AND INFECTION, 2022, 28 (06) : 880.e1 - 880.e8
  • [43] Epidemiology and high incidence of Metallo-β-lactamase and AmpC-β-lactamases in nosocomial Pseudomonas aeruginosa
    Muddassir, Maria
    Munir, Sadaf
    Raza, Almas
    Basirat, Ahmad
    Ahmed, Muddassir
    Farooq, Umar
    Ahmed, Syed Shoaib
    Naqvi, Syed Zeeshan Haider
    IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 2021, 24 (10) : 1373 - 1379
  • [44] Metallo-β-lactamase producing Pseudomonas aeruginosa strains isolated in hospitals in recife, PE, Brazil
    Magalhaes, V
    Lins, AK
    Magalhaes, M
    BRAZILIAN JOURNAL OF MICROBIOLOGY, 2005, 36 (02) : 123 - 125
  • [45] Biochemical characterization of the acquired metallo-β-lactamase SPM-1 from Pseudomonas aeruginosa
    Murphy, TA
    Simm, AM
    Toleman, MA
    Jones, RN
    Walsh, TR
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (02) : 582 - 587
  • [46] Prospective evaluation of imipenem/EDTA combined disc and Etest for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa
    Berges, L.
    Rodriguez-Villalobos, H.
    Deplano, A.
    Struelens, M. J.
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 59 (04) : 812 - 813
  • [47] Carbapenem-resistant Pseudomonas aeruginosa with acquired blaVIM metallo-β-lactamase determinants, Italy
    Rossolini, GM
    Riccio, ML
    Cornaglia, G
    Pagani, L
    Lagatolla, C
    Selan, L
    Fontana, R
    EMERGING INFECTIOUS DISEASES, 2000, 6 (03) : 312 - 313
  • [48] Diversity of β-Lactamases in Pseudomonas aeruginosa Isolates Producing Metallo-β-Lactamase in Two Tunisian Hospitals
    Ktari, Sonia
    Mnif, Basma
    Znazen, Abir
    Rekik, Manel
    Mezghani, Sonda
    Mahjoubi-Rhimi, Fouzia
    Hammami, Adnane
    MICROBIAL DRUG RESISTANCE, 2011, 17 (01) : 25 - 30
  • [49] Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from Southern Brazil
    Wirth, Fernanda W.
    Picoli, Simoni U.
    Cantarelli, Vlademir V.
    Goncalves, Ana L. S.
    Brust, Flavia R.
    Santos, Liege M. O.
    Barreto, Michelle F.
    BRAZILIAN JOURNAL OF INFECTIOUS DISEASES, 2009, 13 (03): : 170 - 172
  • [50] Dissemination of metallo-β-lactamase in Pseudomonas aeruginosa isolates in Egypt: mutation in blaVIM-4
    Hashem, Hany
    Hanora, Amro
    Abdalla, Salah
    Shaeky, Alaa
    Saad, Alaa
    APMIS, 2017, 125 (05) : 499 - 505