Simple microdilution test for detection of metallo-β-lactamase production in Pseudomonas aeruginosa

被引：70
|
作者：
Migliavacca, R
Docquier, JD
Mugnaioli, C
Amicosante, G
Daturi, R
Lee, KW
Rossolini, GM
Pagani, L
机构：
[1] Univ Pavia, Sez Nucribuik, Dipartimento Sci Morfol Eidol & Clin, I-27100 Pavia, Italy
[2] IRCCS S Matteo, Serv Anal Microbiol, I-27100 Pavia, Italy
[3] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy
[4] Univ Aquila, Dipartimento Sci & Tecnol BIomed, I-67100 Laquila, Italy
[5] Yonsei Univ, Coll Med, Dept Clin Pathol, Seoul 120749, South Korea
[6] Yonsei Univ, Coll Med, Res Inst Bacterial Resistance, Seoul 120749, South Korea
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 11期
关键词：
D O I：
10.1128/JCM.40.11.4388-4390.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18VIM- and 4 IMP-type metallo-beta-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.
引用
收藏
页码：4388 / 4390
页数：3
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