Simple microdilution test for detection of metallo-β-lactamase production in Pseudomonas aeruginosa

被引:70
|
作者
Migliavacca, R
Docquier, JD
Mugnaioli, C
Amicosante, G
Daturi, R
Lee, KW
Rossolini, GM
Pagani, L
机构
[1] Univ Pavia, Sez Nucribuik, Dipartimento Sci Morfol Eidol & Clin, I-27100 Pavia, Italy
[2] IRCCS S Matteo, Serv Anal Microbiol, I-27100 Pavia, Italy
[3] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy
[4] Univ Aquila, Dipartimento Sci & Tecnol BIomed, I-67100 Laquila, Italy
[5] Yonsei Univ, Coll Med, Dept Clin Pathol, Seoul 120749, South Korea
[6] Yonsei Univ, Coll Med, Res Inst Bacterial Resistance, Seoul 120749, South Korea
关键词
D O I
10.1128/JCM.40.11.4388-4390.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18VIM- and 4 IMP-type metallo-beta-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.
引用
收藏
页码:4388 / 4390
页数:3
相关论文
共 50 条
  • [31] Detection of Pseudomonas aeruginosa producing metallo-β-lactamase VIM-2 in a central hospital from Portugal
    A. Pena
    A. M. Donato
    A. F. Alves
    R. Leitão
    O. M. Cardoso
    European Journal of Clinical Microbiology & Infectious Diseases, 2008, 27 : 1269 - 1271
  • [32] Molecular Detection of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa and Pseudomonas fluorescence Clinical Isolates in Khartoum State, Sudan
    Osman, Najem Aldin M.
    Hashim, Ammar M.
    Abuzeid, Nadir
    Altayb, Hisham
    Eldirdery, Muataz Mohmed
    Elwakeel, Ali Suleiman
    Eleragi, Ali Mohamed Elhassan
    PAKISTAN JOURNAL OF MEDICAL & HEALTH SCIENCES, 2020, 14 (02): : 1011 - 1014
  • [33] Comparison of disc and MIC reduction methods with polymerase chain reaction for the detection of metallo-β-lactamase in Pseudomonas aeruginosa
    Buchunde, S.
    Mendiratta, D. K.
    Deotale, V.
    Narang, P.
    INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2012, 30 (02) : 170 - 174
  • [34] Metallo-β-Lactamase Production by Pseudomonas otitidis: a Species-Related Trait
    Thaller, Maria Cristina
    Borgianni, Luisa
    Di Lallo, Gustavo
    Chong, Yunsop
    Lee, Kyungwon
    Dajcs, Joseph
    Stroman, David
    Rossolini, Gian Maria
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (01) : 118 - 123
  • [35] A simple test for the detection of KPC and metallo-β-lactamase carbapenemase-producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin
    Pasteran, F.
    Veliz, O.
    Faccone, D.
    Guerriero, L.
    Rapoport, M.
    Mendez, T.
    Corso, A.
    CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (09) : 1438 - 1441
  • [36] In Vitro Potentiation of Carbapenems with ME1071, a Novel Metallo-β-Lactamase Inhibitor, against Metallo-β-Lactamase-Producing Pseudomonas aeruginosa Clinical Isolates
    Ishii, Yoshikazu
    Eto, Maki
    Mano, Yoko
    Tateda, Kazuhiro
    Yamaguchi, Keizo
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (09) : 3625 - 3629
  • [37] Carbapenem-hydrolyzing metallo-β-lactamase from a nosocomial isolate of Pseudomonas aeruginosa in France
    Poirel, L
    Collet, L
    Nordmann, P
    EMERGING INFECTIOUS DISEASES, 2000, 6 (01) : 84 - 85
  • [38] Detection of metallo-β-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran
    Khosravi, Azar D.
    Mihani, Fatemeh
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2008, 60 (01) : 125 - 128
  • [39] VIM-2 metallo-β-lactamase emerges in Pseudomonas aeruginosa isolated from India
    Toleman, M. A.
    Vinodh, H.
    Sekar, U.
    Vijaylakshmi, K.
    Walsh, T. R.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2007, 29 : S107 - S107
  • [40] Emergence of a New Delhi metallo-β-lactamase-1-producing Pseudomonas aeruginosa in Singapore
    Teo, Jeanette W. P.
    La, My-Van
    Jureen, Roland
    Lin, Raymond T. P.
    EMERGING MICROBES & INFECTIONS, 2015, 4