Cloning, characterization and expression of β-N-acetylglucosaminidase gene from Streptomyces thermoviolaceus OPC-520

被引：14
|
作者：
Tsujibo, H [1 ]
Hatano, N [1 ]
Mikami, T [1 ]
Izumizawa, Y [1 ]
Miyamoto, K [1 ]
Inamori, Y [1 ]
机构：
[1] Osaka Univ Pharmaceut Sci, Osaka 5691094, Japan
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1998年 / 1425卷 / 02期
关键词：
NagB; chitinolytic enzyme; (Streptomyces thermoviolaceus OPC-520);
D O I：
10.1016/S0304-4165(98)00098-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The nagB gene encoding beta-N-acetylglucosaminidase from S. thermoviolaceus OPC-520 was cloned and sequenced. The nagB gene could encode a protein of 541 amino acids with a calculated molecular mass of 58274. NagB revealed significant similarities to beta-N-acetylhexosaminidases and chitobiases from bacteria, which are classified into family 20 glycosyl hydrolases. NagB effectively hydrolyzed all of the chitin oligosaccharides from dimer to hexamer. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：437 / 440
页数：4
相关论文
共 50 条
  • [31] Purification and characterization of N-acetylglucosaminidase from Trypanosoma cruzi
    ElMoudni, B
    Rodier, MH
    Jacquemin, JL
    EXPERIMENTAL PARASITOLOGY, 1996, 83 (02) : 167 - 173
  • [32] Cloning, nucleotide sequence and expression of the β-N-acetylglucosaminidase gene from Aeromonas sp no. 10S-24
    Ueda, M
    Fujita, Y
    Kawaguchi, T
    Arai, M
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2000, 89 (02) : 164 - 169
  • [33] Purification and Characterization of β-N-acetylglucosaminidase from Grifola frondosa
    Wang, Yi-Cheng
    Lien, Te-Sheng
    Chen, Nan-Yin
    Hsu, Tai-Hao
    BIORESOURCES, 2021, 16 (04) : 7233 - 7247
  • [34] Molecular cloning and functional characterization of β-N-acetylglucosaminidase genes from Sf9 cells
    Aumiller, Jared J.
    Hollister, Jason R.
    Jarvis, Donald L.
    PROTEIN EXPRESSION AND PURIFICATION, 2006, 47 (02) : 571 - 590
  • [35] Purification, Characterization, and Gene Expression of Rice Endo-β-N-Acetylglucosaminidase, Endo-Os
    Maeda, Megumi
    Okamoto, Naoko
    Araki, Node
    Kimura, Yoshinobu
    FRONTIERS IN PLANT SCIENCE, 2021, 12
  • [36] Purification, characterization and molecular cloning of a novel endo-β-N-acetylglucosaminidase from the basidiomycete, Flammulina velutipes
    Hamaguchi, Tasuku
    Ito, Tsukasa
    Inoue, Yukako
    Limpaseni, Tipaporn
    Pongsawasdi, Piamsook
    Ito, Kazuo
    GLYCOBIOLOGY, 2010, 20 (04) : 420 - 432
  • [37] Characterization of AcmB, an N-acetylglucosaminidase autolysin from Lactococcus lactis
    Huard, C
    Miranda, G
    Wessner, FO
    Bolotin, A
    Hansen, J
    Foster, SJ
    Chapot-Chartier, MP
    MICROBIOLOGY-SGM, 2003, 149 : 695 - 705
  • [38] Purification, characterization and gene analysis of N-acetylglucosaminidase from Enterobacter sp G-1
    Matsuo, Y
    Kurita, M
    Park, JK
    Tanaka, K
    Nakagawa, T
    Kawamukai, M
    Matsuda, H
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (07) : 1261 - 1268
  • [39] Characterization of a β-N-acetylglucosaminidase gene and its involvement in the development of Lasioderma serricorne (Fabricius)
    Chen, Xiaoyulong
    Xu, Kangkang
    Yan, Xin
    Chen, Chunxu
    Cao, Yu
    Wang, Yawei
    Li, Can
    Yang, Wenjia
    JOURNAL OF STORED PRODUCTS RESEARCH, 2018, 77 : 156 - 165
  • [40] Characterization of a β-N-acetylhexosaminidase and a β-N-acetylglucosaminidase/β-glucosidase from Cellulomonas fimi
    Mayer, C
    Vocadlo, DJ
    Mah, M
    Rupitz, K
    Stoll, D
    Warren, RAJ
    Withers, SG
    FEBS JOURNAL, 2006, 273 (13) : 2929 - 2941
12345