Cloning, characterization and expression of β-N-acetylglucosaminidase gene from Streptomyces thermoviolaceus OPC-520

被引：14
|
作者：
Tsujibo, H [1 ]
Hatano, N [1 ]
Mikami, T [1 ]
Izumizawa, Y [1 ]
Miyamoto, K [1 ]
Inamori, Y [1 ]
机构：
[1] Osaka Univ Pharmaceut Sci, Osaka 5691094, Japan
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1998年 / 1425卷 / 02期
关键词：
NagB; chitinolytic enzyme; (Streptomyces thermoviolaceus OPC-520);
D O I：
10.1016/S0304-4165(98)00098-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The nagB gene encoding beta-N-acetylglucosaminidase from S. thermoviolaceus OPC-520 was cloned and sequenced. The nagB gene could encode a protein of 541 amino acids with a calculated molecular mass of 58274. NagB revealed significant similarities to beta-N-acetylhexosaminidases and chitobiases from bacteria, which are classified into family 20 glycosyl hydrolases. NagB effectively hydrolyzed all of the chitin oligosaccharides from dimer to hexamer. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：437 / 440
页数：4
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