Cloning, nucleotide sequence and expression of the β-N-acetylglucosaminidase gene from Aeromonas sp no. 10S-24

被引：10
|
作者：
Ueda, M [1 ]
Fujita, Y [1 ]
Kawaguchi, T [1 ]
Arai, M [1 ]
机构：
[1] Univ Osaka Prefecture, Coll Agr, Dept Appl Biol Chem, Sakai, Osaka 5998531, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2000年 / 89卷 / 02期
关键词：
beta-GlcNAcase; Aeromonas; chitinase; cloning; expression; carbohydrate chain;
D O I：
10.1016/S1389-1723(00)88731-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The beta-GlcNAcase gene of Aeromonas sp. no. 10S-24 was cloned and sequenced. The gene consisted of 2505 bp encoding 835 amino acid residues. The amino acid sequence of the gene shares sequence homology with beta-GlcNAcases of Vibrio vulnificus (37.4%), Serratia marcescens (36.4%), V. harveyi (36.4%), V, para-hemolyticus (33.5%), Alteromonas sp, strain O-7 (27.0%), and Candida albicans (28.6%). The beta-GlcNAcase gene of Aeromonas sp. no. 10S-24 was about 630-fold overexpressed in Escherichia coli compared to that in Aeromonas sp. no. 10S-24, The cloned beta-GlcNAcase had almost the same enzymatic properties as beta-GlcNAcase from no. 10S-24. The carbohydrate chain of beta-GlcNAcase from Aeromonas sp, no. 10S-24 was found to contain mannose, galactose, fucose, N-acetylglucosamine, N-acetylgalactosamine and several other unknown saccharides.
引用
收藏
页码：164 / 169
页数：6
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