The application of FPLC chromatography (Mono Q) for the purification of wheat RNA polymerases II and III

被引：0
|
作者：
Glanc, P
Galbas, M
Dullin, P
Szalata, M
Slomski, R
机构：
[1] Univ Agr, Dept Biochem & Biotechnol, PL-60637 Poznan, Poland
[2] Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland
来源：
CELLULAR & MOLECULAR BIOLOGY LETTERS | 2003年 / 8卷 / 03期
关键词：
wheat; transcription; RNA polymerase II and III; Mono Q column; FPLC;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transcription is the main step in the regulation of gene expression. To study this process in vitro, it is necessary to obtain highly purified RNA polymerases. Here, we describe a method of RNA polymerase purification using a Mono Q FPLC column. Using Mono Q column chromatography accelerates the purification process and separates RNA polymerase 11 from RNA polymerase III with good yield.
引用
收藏
页码：825 / 830
页数：6
相关论文
共 50 条
  • [41] RNA POLYMERASES I, II AND III OF KB CELLS AND FIRST PURINE NUCLEOTIDE USED IN SYNTHESIS OF RNA IN-VITRO
    VERBERT, AP
    SERGEANT, A
    DHALLUIN, JC
    ARCHIVES INTERNATIONALES DE PHYSIOLOGIE DE BIOCHIMIE ET DE BIOPHYSIQUE, 1973, 81 (03): : 601 - 601
  • [42] APPLICATION OF COUNTER ALTERNATIVE CURRENT CHROMATOGRAPHY TO PURIFICATION OF FOOD MONO-AZO DYES
    KABASAWA, Y
    TANIMURA, T
    NAKAZAWA, H
    SHINOMIYA, K
    ANALYTICAL SCIENCES, 1992, 8 (03) : 351 - 353
  • [43] Application of steric exclusion chromatography on monoliths for separation and purification of RNA molecules
    Levanova, Alesia
    Poranen, Minna M.
    JOURNAL OF CHROMATOGRAPHY A, 2018, 1574 : 50 - 59
  • [44] Human Cytomegalovirus Infection Elicits Global Changes in Host Transcription by RNA Polymerases I, II, and III
    Ball, Christopher B.
    Parida, Mrutyunjaya
    Li, Ming
    Spector, Benjamin M.
    Suarez, Gustavo A.
    Meier, Jeffery L.
    Price, David H.
    VIRUSES-BASEL, 2022, 14 (04):
  • [45] Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III
    Jones, E
    Kimura, H
    Vigneron, M
    Wang, ZX
    Roeder, RG
    Cook, PR
    EXPERIMENTAL CELL RESEARCH, 2000, 254 (01) : 163 - 172
  • [46] Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast
    Hamada, M
    Huang, Y
    Lowe, TM
    Maraia, RJ
    MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (20) : 6870 - 6881
  • [47] APPLICATION OF AFFINITY CHROMATOGRAPHY IN PURIFICATION OF ENZYMATIC COMPLEXES - MITOCHONDRIAL COMPLEX-III
    TESTOLIN, G
    BRENNA, O
    CIAPPELLANO, S
    PIETTA, PG
    PACE, M
    ITALIAN JOURNAL OF BIOCHEMISTRY, 1977, 26 (01): : 84 - 84
  • [48] WHEAT LEAF RNA POLYMERASES .1. PARTIAL PURIFICATION AND CHARACTERIZATION OF NUCLEAR, CHLOROPLAST AND SOLUBLE DNA-DEPENDENT ENZYMES
    POLYA, GM
    JAGENDOR.AT
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1971, 146 (02) : 635 - &
  • [49] Expression and purification of recombinant hRPABC25, hRPABC17, and hRPABC14.4, three essential subunits of human RNA polymerases I, II, and III
    Hiremath, CN
    Ladias, JAA
    PROTEIN EXPRESSION AND PURIFICATION, 1998, 13 (02) : 198 - 204
  • [50] APPLICATION OF IMMOBILIZED METAL AFFINITY-CHROMATOGRAPHY FOR PURIFICATION OF WHEAT ALPHA-AMYLASES
    ZAWISTOWSKA, U
    SANGSTER, K
    FRIESEN, AD
    CEREAL FOODS WORLD, 1987, 32 (09) : 671 - 671
12345