The application of FPLC chromatography (Mono Q) for the purification of wheat RNA polymerases II and III

被引：0

作者：

Glanc, P

Galbas, M

Dullin, P

Szalata, M

Slomski, R

机构：

[1] Univ Agr, Dept Biochem & Biotechnol, PL-60637 Poznan, Poland

[2] Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland

来源：

CELLULAR & MOLECULAR BIOLOGY LETTERS | 2003年 / 8卷 / 03期

关键词：

wheat; transcription; RNA polymerase II and III; Mono Q column; FPLC;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription is the main step in the regulation of gene expression. To study this process in vitro, it is necessary to obtain highly purified RNA polymerases. Here, we describe a method of RNA polymerase purification using a Mono Q FPLC column. Using Mono Q column chromatography accelerates the purification process and separates RNA polymerase 11 from RNA polymerase III with good yield.

引用

页码：825 / 830

页数：6

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