The application of FPLC chromatography (Mono Q) for the purification of wheat RNA polymerases II and III

被引：0
|
作者：
Glanc, P
Galbas, M
Dullin, P
Szalata, M
Slomski, R
机构：
[1] Univ Agr, Dept Biochem & Biotechnol, PL-60637 Poznan, Poland
[2] Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland
来源：
CELLULAR & MOLECULAR BIOLOGY LETTERS | 2003年 / 8卷 / 03期
关键词：
wheat; transcription; RNA polymerase II and III; Mono Q column; FPLC;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transcription is the main step in the regulation of gene expression. To study this process in vitro, it is necessary to obtain highly purified RNA polymerases. Here, we describe a method of RNA polymerase purification using a Mono Q FPLC column. Using Mono Q column chromatography accelerates the purification process and separates RNA polymerase 11 from RNA polymerase III with good yield.
引用
收藏
页码：825 / 830
页数：6
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