Preparation and characterization of 125 I labeled bovine serum albumin

被引:0
|
作者
Rai, K. S. Ashwitha [1 ]
Jyothi [1 ]
Rasmi, R. R. [1 ]
Sarnaik, Jayula [2 ]
Kadwad, V. B. [2 ]
Shenoy, K. B. [1 ]
Somashekarappa, H. M. [1 ]
机构
[1] Mangalore Univ, CARRT, Mangalagangothri 574199, Karnataka, India
[2] BRIT, Radiopharmaceut Prod Programme, Vashi 400705, Navi Mumbai, India
关键词
Bovine serum albumin; chloramine-T; radiolabeled; tracer; RADIOIODINATION; PROTEINS;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ( (125) I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 mu g of chloramine-T along with 20 mu g of sodium metabisulphite with 60 seconds incubation at 2 degrees yielded (125) I-BSA with high integrity.
引用
收藏
页码:107 / 110
页数:4
相关论文
共 50 条
  • [2] EXPERIENCE WITH A COMMERCIAL PREPARATION OF I-125-LABELED HUMAN ALBUMIN FOR STUDY OF ALBUMIN METABOLISM
    BALLANTYNE, FC
    FLECK, A
    JOURNAL OF CLINICAL PATHOLOGY, 1973, 26 (07) : 499 - 502
  • [3] Preparation and characterization of molecularly imprinted polymer of bovine serum albumin
    Zhang, Jing
    Ma, Jinqin
    Yue, Xuanfeng
    Bu, Xinli
    Han, Yanting
    JOURNAL OF APPLIED POLYMER SCIENCE, 2012, 124 (01) : 723 - 728
  • [4] Preparation and Characterization of Chitosan Microsphere Loading Bovine Serum Albumin
    孙庆申
    付宏刚
    JournalofWuhanUniversityofTechnology(MaterialsScienceEdition), 2012, 27 (03) : 459 - 464
  • [5] Preparation and characterization of chitosan microsphere loading bovine serum albumin
    Sun Qingshen
    Han Dequan
    Lei Hong
    Zhao Kai
    Zhu Li
    Li Xiaodi
    Fu Honggang
    JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION, 2012, 27 (03): : 459 - 464
  • [6] Preparation and characterization of a bovine serum albumin-catalase conjugate
    Tao Hu
    Zhiguo Su
    Biotechnology Letters, 2002, 24 : 1027 - 1030
  • [7] Preparation and characterization of chitosan microsphere loading bovine serum albumin
    Qingshen Sun
    Dequan Han
    Hong Lei
    Kai Zhao
    Li Zhu
    Xiaodi Li
    Honggang Fu
    Journal of Wuhan University of Technology-Mater. Sci. Ed., 2012, 27 : 459 - 464
  • [8] Preparation and characterization of a bovine serum albumin-catalase conjugate
    Hu, T
    Su, ZG
    BIOTECHNOLOGY LETTERS, 2002, 24 (12) : 1027 - 1030
  • [9] Preparation and Characterization of Improved Microspheres Containing Bovine Serum Albumin
    Li, Fan
    Tian, Feng
    Liu, Chang-Jun
    Zhao, Yong-Liang
    JOURNAL OF APPLIED POLYMER SCIENCE, 2009, 114 (02) : 818 - 825
  • [10] Preparation and characterization of bisphenol A-cationized bovine serum albumin
    Feng, Yi
    Zhou, Youxiang
    Zou, Qiang
    Wang, Jixing
    Chen, Fusheng
    Gao, Zhixian
    JOURNAL OF IMMUNOLOGICAL METHODS, 2009, 340 (02) : 138 - 143