Preparation and characterization of 125 I labeled bovine serum albumin

Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ( (125) I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 mu g of chloramine-T along with 20 mu g of sodium metabisulphite with 60 seconds incubation at 2 degrees yielded (125) I-BSA with high integrity.