Preparation and characterization of 125 I labeled bovine serum albumin

被引：0

作者：

Rai, K. S. Ashwitha ^{[1
]}

Jyothi ^{[1
]}

Rasmi, R. R. ^{[1
]}

Sarnaik, Jayula ^{[2
]}

Kadwad, V. B. ^{[2
]}

Shenoy, K. B. ^{[1
]}

Somashekarappa, H. M. ^{[1
]}

机构：

[1] Mangalore Univ, CARRT, Mangalagangothri 574199, Karnataka, India

[2] BRIT, Radiopharmaceut Prod Programme, Vashi 400705, Navi Mumbai, India

来源：

INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2015年 / 77卷 / 01期

关键词：

Bovine serum albumin; chloramine-T; radiolabeled; tracer; RADIOIODINATION; PROTEINS;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ( (125) I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 mu g of chloramine-T along with 20 mu g of sodium metabisulphite with 60 seconds incubation at 2 degrees yielded (125) I-BSA with high integrity.

引用

页码：107 / 110

页数：4

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