Preparation and characterization of a bovine serum albumin-catalase conjugate

被引：1
|
作者：
Hu, T [1 ]
Su, ZG [1 ]
机构：
[1] Chinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 12期
基金：
中国国家自然科学基金;
关键词：
bovine serum albumin; catalase; O-2 free radicals; N-succinimidyl 3-(2-pyridyldithio)propionate;
D O I：
10.1023/A:1015641416286
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A bovine serum albumin-catalase conjugate was prepared using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The purified conjugate had an M-r of 380 kDa. The half-life time of the conjugate was 6, 69 and 65 min after heat treatment, urea treatment and trypsin hydrolysis, respectively, whereas that of native catalase was 3, 22, 28 min, respectively.
引用
收藏
页码：1027 / 1030
页数：4
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