Development and validation of reversed phase HPLC method for the estimation of nevirapine in pure form and in pharmaceutical dosage forms

被引：0
|
作者：
Sankar, DG [1 ]
Kumar, DVSP [1 ]
机构：
[1] Andhra Univ, Dept Pharmaceut Sci, Visakhapatnam 530003, Andhra Pradesh, India
来源：
ASIAN JOURNAL OF CHEMISTRY | 2005年 / 17卷 / 04期
关键词：
RP-HPLC; nevirapine tablets;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A simple, precise reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the estimation of nevirapine in bulk as well as in pharmaceutical dosage forms. The quantification was carried out using a BDS Hypersil C-18 column 250 mm x 4.6 mm i.d., 5 gm particle size in isocratic mode, with mobile phase comprising phosphate buffer and acetonitrile in the ratio of 75 : 25 (v/v). The flow rate was 1.5 mL/min and the detection was carried out at 220 nm. The retention time was 6.748 min. The method produced linear response in the concentration range of 60-130 mu g/mL and the percentage recovery ranged from 98.15-99.01.
引用
收藏
页码：2575 / 2578
页数：4
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