DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF RITONAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS

A simple, precise, specific and accurate reverse phase HPLC method has been developed for the determination of Ritonavir in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved on Symmetry C18 (4.6 x 100mm, 3.5 mu m) column using a mixture of Buffer: Acetonitrile (50:50) as the mobile phase at a flow rate 1.0 ml/min. Linearity was observed in concentration range of 50-150 mu g/ml. The retention time of Ritonavir was 5.1 min. The analyte was monitored using UV detector at 239 nm. Results of analysis were validated statistically and by recovery studies. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness.