Development and validation of reversed phase HPLC method for the estimation of nevirapine in pure form and in pharmaceutical dosage forms

A simple, precise reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the estimation of nevirapine in bulk as well as in pharmaceutical dosage forms. The quantification was carried out using a BDS Hypersil C-18 column 250 mm x 4.6 mm i.d., 5 gm particle size in isocratic mode, with mobile phase comprising phosphate buffer and acetonitrile in the ratio of 75 : 25 (v/v). The flow rate was 1.5 mL/min and the detection was carried out at 220 nm. The retention time was 6.748 min. The method produced linear response in the concentration range of 60-130 mu g/mL and the percentage recovery ranged from 98.15-99.01.