Microscopy with microlens arrays: high throughput, high resolution and light-field imaging

被引:72
|
作者
Orth, Antony [1 ]
Crozier, Kenneth [1 ]
机构
[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
来源
OPTICS EXPRESS | 2012年 / 20卷 / 12期
基金
美国国家科学基金会;
关键词
OF-VIEW MICROSCOPE; FABRICATION; DESIGN;
D O I
10.1364/OE.20.013522
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We demonstrate highly parallelized fluorescence scanning microscopy using a refractive microlens array. Fluorescent beads and rat femur tissue are imaged over a 5.5 mm x 5.5 mm field of view at a pixel throughput of up to 4 megapixels/s and a resolution of 706 nm. We also demonstrate the ability to extract different perspective views of a pile of microspheres. (C) 2012 Optical Society of America
引用
收藏
页码:13522 / 13531
页数:10
相关论文
共 50 条
  • [1] Light-field microscopy with correlated beams for high-resolution volumetric imaging
    Massaro, Gianlorenzo
    Giannella, Davide
    Scagliola, Alessio
    Lena, Francesco Di
    Scarcelli, Giuliano
    Garuccio, Augusto
    Pepe, Francesco V.
    D'Angelo, Milena
    SCIENTIFIC REPORTS, 2022, 12 (01)
  • [2] Light-field microscopy with correlated beams for high-resolution volumetric imaging
    Gianlorenzo Massaro
    Davide Giannella
    Alessio Scagliola
    Francesco Di Lena
    Giuliano Scarcelli
    Augusto Garuccio
    Francesco V. Pepe
    Milena D’Angelo
    Scientific Reports, 12
  • [3] High throughput multichannel fluorescence microscopy with microlens arrays
    Orth, Antony
    Crozier, Kenneth B.
    OPTICS EXPRESS, 2014, 22 (15): : 18101 - 18112
  • [4] High-resolution light-field microscopy with patterned illumination
    Wang, Depeng
    Roy, Suva
    Rudzite, Andra M.
    Field, Greg D.
    Gong, Yiyang
    BIOMEDICAL OPTICS EXPRESS, 2021, 12 (07): : 3887 - 3901
  • [5] High-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy
    Liu, Wenhao
    Jia, Shu
    THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XXIX, 2022, 11966
  • [6] Fast, volumetric live-cell imaging using high-resolution light-field microscopy
    Li, Haoyu
    Guo, Changliang
    Kim-Holzapfel, Deborah
    Li, Weiyi
    Altshuller, Yelena
    Schroeder, Bryce
    Liu, Wenhao
    Meng, Yizhi
    French, Jarrod B.
    Takamaru, Ken-Ichi
    Frohman, Michael A.
    Jia, Shu
    BIOMEDICAL OPTICS EXPRESS, 2019, 10 (01) : 29 - 49
  • [7] Virtual-scanning light-field microscopy for robust snapshot high-resolution volumetric imaging
    Zhi Lu
    Yu Liu
    Manchang Jin
    Xin Luo
    Huanjing Yue
    Zian Wang
    Siqing Zuo
    Yunmin Zeng
    Jiaqi Fan
    Yanwei Pang
    Jiamin Wu
    Jingyu Yang
    Qionghai Dai
    Nature Methods, 2023, 20 : 735 - 746
  • [8] Virtual-scanning light-field microscopy for robust snapshot high-resolution volumetric imaging
    Lu, Zhi
    Liu, Yu
    Jin, Manchang
    Luo, Xin
    Yue, Huanjing
    Wang, Zian
    Zuo, Siqing
    Zeng, Yunmin
    Fan, Jiaqi
    Pang, Yanwei
    Wu, Jiamin
    Yang, Jingyu
    Dai, Qionghai
    NATURE METHODS, 2023, 20 (05) : 735 - +
  • [9] High-Quality Light Field Microscope Imaging Based on Microlens Arrays
    Gu, Tongkai
    Yan, Sitong
    Wang, Lanlan
    Chang, Yasheng
    Liu, Hongzhong
    JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 2024, 33 (02) : 296 - 303
  • [10] High-resolution 3D light-field imaging
    Geng, Qiang
    Fu, Zhiqiang
    Chen, Shih-Chi
    JOURNAL OF BIOMEDICAL OPTICS, 2020, 25 (10)