High throughput multichannel fluorescence microscopy with microlens arrays

被引：26
|
作者：
Orth, Antony [1 ,2 ]
Crozier, Kenneth B. [2 ,3 ,4 ]
机构：
[1] Rowland Inst Harvard, Cambridge, MA 02142 USA
[2] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
[3] Univ Melbourne, Sch Phys, Parkville, Vic 3010, Australia
[4] Univ Melbourne, Dept Elect & Elect Engn, Parkville, Vic 3010, Australia
来源：
OPTICS EXPRESS | 2014年 / 22卷 / 15期
基金：
美国国家科学基金会;
关键词：
WIDE; ILLUMINATION;
D O I：
10.1364/OE.22.018101
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We present a multichannel fluorescence microscopy technique for high throughput imaging applications. A microlens array with over 140,000 elements is used to image centimeter-scale samples at up to 18.1 megapixels per second. Large field-of-view multichannel fluorescent imaging is demonstrated in both sequential and parallel geometries. The extended dynamic range of this approach is also discussed. (C) 2014 Optical Society of America
引用
收藏
页码：18101 / 18112
页数：12
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