Microscopy with microlens arrays: high throughput, high resolution and light-field imaging

被引:72
|
作者
Orth, Antony [1 ]
Crozier, Kenneth [1 ]
机构
[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
来源
OPTICS EXPRESS | 2012年 / 20卷 / 12期
基金
美国国家科学基金会;
关键词
OF-VIEW MICROSCOPE; FABRICATION; DESIGN;
D O I
10.1364/OE.20.013522
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We demonstrate highly parallelized fluorescence scanning microscopy using a refractive microlens array. Fluorescent beads and rat femur tissue are imaged over a 5.5 mm x 5.5 mm field of view at a pixel throughput of up to 4 megapixels/s and a resolution of 706 nm. We also demonstrate the ability to extract different perspective views of a pile of microspheres. (C) 2012 Optical Society of America
引用
收藏
页码:13522 / 13531
页数:10
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