A new interference-free lysine biosensor using a non-conducting polymer film

被引：43

作者：

Curulli, A

Kelly, S

O'Sullivan, C

Guilbault, GG

Palleschi, G

机构：

[1] Studio Elettrochim & Chim Fis Interfasi, Ctr CNR, I-00161 Rome, Italy

[2] Natl Univ Ireland Univ Coll Cork, Cork, Ireland

[3] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, Rome, Italy

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 12期

关键词：

non-conducting polymers; electropolymerisation; interferences; lysine; food analysis; lysine oxidase;

D O I：

10.1016/S0956-5663(98)00075-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An electrochemical biosensor for the determination of lysine to be used for rapid evaluation of food quality has been developed. Platinum electrodes have been coated by electropolymerisation with 1,2-diaminobenzene( 1.2-DAB) using cyclic voltammetry. The reduction in the oxidation of interferents compared with the bare platinum electrode was 100% for ascorbic acid, 99% for acetaminophen and 99% for cysteine. The enzyme L-lysine-cu-oxidase was then immobilised onto the polymer layer by passive adsorption and a calibration curve for lysine constructed. This gave a linear range of 1 x 10(-5) mol/l to 1 x 10(-3) mol/l and a limit of detection of 2 x 10(-7) mel/l. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：1245 / 1250

页数：6

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