A new interference-free lysine biosensor using a non-conducting polymer film

被引：43
|
作者：
Curulli, A
Kelly, S
O'Sullivan, C
Guilbault, GG
Palleschi, G
机构：
[1] Studio Elettrochim & Chim Fis Interfasi, Ctr CNR, I-00161 Rome, Italy
[2] Natl Univ Ireland Univ Coll Cork, Cork, Ireland
[3] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, Rome, Italy
来源：
BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 12期
关键词：
non-conducting polymers; electropolymerisation; interferences; lysine; food analysis; lysine oxidase;
D O I：
10.1016/S0956-5663(98)00075-X
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
An electrochemical biosensor for the determination of lysine to be used for rapid evaluation of food quality has been developed. Platinum electrodes have been coated by electropolymerisation with 1,2-diaminobenzene( 1.2-DAB) using cyclic voltammetry. The reduction in the oxidation of interferents compared with the bare platinum electrode was 100% for ascorbic acid, 99% for acetaminophen and 99% for cysteine. The enzyme L-lysine-cu-oxidase was then immobilised onto the polymer layer by passive adsorption and a calibration curve for lysine constructed. This gave a linear range of 1 x 10(-5) mol/l to 1 x 10(-3) mol/l and a limit of detection of 2 x 10(-7) mel/l. (C) 1998 Elsevier Science S.A. All rights reserved.
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页码：1245 / 1250
页数：6
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