Quencher-free molecular beacon: Enhancement of the signal-to-background ratio with graphene oxide

被引：25
|
作者：
Yi, Jeong Wu [2 ]
Park, Jaesung [1 ]
Singh, N. Jiten [1 ]
Lee, Il Joon [2 ]
Kim, Kwang S. [1 ]
Kim, Byeang Hyean [2 ]
机构：
[1] Pohang Univ Sci & Technol, BK Sch Mol Sci, Dept Chem, Ctr Superfunct Mat, Pohang, South Korea
[2] Pohang Univ Sci & Technol, BK Sch Mol Sci, Dept Chem, Lab Modified Nucle Acid Syst, Pohang 790784, South Korea
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2011年 / 21卷 / 02期
关键词：
Quencher-free molecular beacon; Molecular beacon; Graphene oxide; Signal-to-background ratio; Fluorescence; NUCLEIC-ACIDS; DNA; FILMS; FLUORESCENCE; PLATFORM; SHEETS; STEM;
D O I：
10.1016/j.bmcl.2010.12.004
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
We report the highly improved version of quencher-free molecular beacon (QF-MB) system by using graphene oxide (GO) as an external quencher. This QF-MB/GO system provided a higher S/B ratio (31,0) relative to that (2,2) of the same system in the absence of GO, while retaining a high selectivity for fully matched over single-base-mismatched targets. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：704 / 706
页数：3
相关论文
共 50 条
  • [31] Intermolecular G-quadruplex-based universal quencher free molecular beacon
    Zhou, Hui
    Xie, Su-Jin
    Li, Ji-Shan
    Wu, Zai-Sheng
    Shen, Guo-Li
    CHEMICAL COMMUNICATIONS, 2012, 48 (87) : 10760 - 10762
  • [32] Quantitative Analysis of Heavy Metals in Water with LIBS Based on Signal-to-Background Ratio
    Hu Li
    Zhao Nan-jing
    Liu Wen-qing
    Fang Li
    Zhang Da-hai
    Wang Yin
    Meng De-shuo
    Yu Yang
    Ma Ming-jun
    SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35 (07) : 2017 - 2020
  • [33] Molecular Beacon Lighting up on Graphene Oxide
    Huang, Po-Jung Jimmy
    Liu, Juewen
    ANALYTICAL CHEMISTRY, 2012, 84 (09) : 4192 - 4198
  • [34] RESOLUTION AND SIGNAL-TO-BACKGROUND ENHANCEMENT IN GAS-PHASE ELECTRON-SPECTROSCOPY
    BALTZER, P
    KARLSSON, L
    LUNDQVIST, M
    WANNBERG, B
    REVIEW OF SCIENTIFIC INSTRUMENTS, 1993, 64 (08): : 2179 - 2189
  • [35] Assembly of Graphene Oxide and Au0.7Ag0.3 Alloy Nanoparticles on SiO2: A New Raman Substrate with Ultrahigh Signal-to-Background Ratio
    Chen, Peng
    Yin, Zongyou
    Huang, Xiao
    Wu, Shixin
    Liedberg, Bo
    Zhang, Hua
    JOURNAL OF PHYSICAL CHEMISTRY C, 2011, 115 (49): : 24080 - 24084
  • [36] A simple PCR-based fluorometric system for detection of mutant fusion DNAs using a quencher-free fluorescent DNA probe and graphene oxide
    Roh, Kyoungmin
    Kim, Dong-Min
    Lee, Eun Hee
    Kim, Hyoseon
    Park, Hyung Soon
    Jang, Ja-Hyun
    Hwang, Sang-Hyun
    Kim, Dong-Eun
    CHEMICAL COMMUNICATIONS, 2015, 51 (32) : 6960 - 6963
  • [37] The impact of iterative reconstruction protocol, signal-to-background ratio and background activity on measurement of PET spatial resolution
    Rezaei, Sahar
    Ghafarian, Pardis
    Bakhshayesh-Karam, Mehrdad
    Uribe, Carlos F.
    Rahmim, Arman
    Sarkar, Saeed
    Ay, Mohammad Reza
    JAPANESE JOURNAL OF RADIOLOGY, 2020, 38 (03) : 231 - 239
  • [38] The impact of iterative reconstruction protocol, signal-to-background ratio and background activity on measurement of PET spatial resolution
    Sahar Rezaei
    Pardis Ghafarian
    Mehrdad Bakhshayesh-Karam
    Carlos F. Uribe
    Arman Rahmim
    Saeed Sarkar
    Mohammad Reza Ay
    Japanese Journal of Radiology, 2020, 38 : 231 - 239
  • [39] Assembly of DNA Probes into Superstructures for Dramatically Enhancing Enzymatic Stability and Signal-to-Background Ratio
    Wang, Fengchao
    Li, Qiang
    Zhang, Pengjuan
    Liu, Xuehui
    Li, Ang
    Yang, Jie
    Liu, Dingbin
    ACS SENSORS, 2018, 3 (12): : 2702 - 2708
  • [40] PLK1-Targeted Fluorescent Tumor Imaging with High Signal-to-Background Ratio
    Hou, Ji-Ting
    Ko, Kyung-Phil
    Shi, Hu
    Ren, Wen Xiu
    Verwilst, Peter
    Koo, Seyoung
    Lee, Jin Yong
    Chi, Sung-Gil
    Kim, Jong Seung
    ACS SENSORS, 2017, 2 (10): : 1512 - 1516
12345