Resveratrol Alleviates Skeletal Muscle Insulin Resistance by Downregulating Long Noncoding RNA

被引:12
|
作者
Liu, Zhihong [1 ,2 ,3 ]
Zhang, Zhimei [1 ,2 ]
Song, Guangyao [1 ,2 ]
Wang, Xing [4 ]
Xing, Hanying [4 ]
Wang, Chao [4 ]
机构
[1] Hebei Med Univ, Dept Internal Med, Shijiazhuang 050017, Hebei, Peoples R China
[2] Hebei Gen Hosp, Dept Endocrinol, Shijiazhuang 050051, Hebei, Peoples R China
[3] Hebei Med Univ, Dept Endocrinol, Second Hosp, Shijiazhuang 050000, Hebei, Peoples R China
[4] Hebei Gen Hosp, Hebei Key Lab Metab Dis, Shijiazhuang 050051, Hebei, Peoples R China
关键词
PATHWAY; CELLS; PROLIFERATION; MECHANISM; INVASION; OBESITY; SOCS-1; AXIS;
D O I
10.1155/2022/2539519
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Long noncoding RNA (lncRNA) is a crucial factor in the progression of insulin resistance (IR). Resveratrol (RSV) exhibits promising therapeutic potential for IR. However, there are few studies on whether RSV improves IR through lncRNA. This study aimed to determine whether RSV could influence the expression of lncRNA and to elucidate the underlying mechanism. Mice were divided into three groups: control group, high-fat diet (HFD) group, and HFD + RSV group. We conducted a high-throughput sequencing analysis to detect lncRNA and mRNA expression signatures and the ceRNA-network in the skeletal muscles of mice that were fed an HFD to induce IR. Hierarchical clustering, gene enrichment, and gene ceRNA-network analyses were subsequently conducted. Differentially expressed lncRNAs were selected and validated via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The biological functions of the selected lncRNAs were investigated by silencing the target genes via lentivirus transfection of C2C12 mouse myotube cells. RSV treatment reversed the expression of 338 mRNAs and 629 lncRNAs in the skeletal muscles of mice with HFD-induced IR. The results of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes database analyses indicated that the differentially expressed mRNAs modulated type II diabetes mellitus. After validating randomly selected lncRNAs via RT-qPCR, we identified a novel lncRNA, NONMMUT044897.2, which was upregulated in the HFD group and reversed with RSV treatment. Additionally, NONMMUT044897.2 was proven to function as a ceRNA of microRNA- (miR-) 7051-5p. Suppressor of Cytokine Signaling 1 (SOCS1) was confirmed as a target of miR-7051-5p. We further performed lentivirus transfection to knock down NONMMUT044897.2 in vitro and found that NONMMUT044897.2 silenced SOCS1 and potentiated the insulin signaling pathway. Hence, RSV mimicked the silencing effect of lentivirus transfection on NONMMUT044897.2. Our study revealed that RSV reduced IR in mouse skeletal muscles via the regulation of NONMMUT044897.2.
引用
收藏
页数:16
相关论文
共 50 条
  • [41] MUNC, a Long Noncoding RNA That Facilitates the Function of MyoD in Skeletal Myogenesis
    Mueller, Adam C.
    Cichewicz, Magdalena A.
    Dey, Bijan K.
    Layer, Ryan
    Reon, Brian J.
    Gagan, Jeffrey R.
    Dutta, Anindya
    MOLECULAR AND CELLULAR BIOLOGY, 2015, 35 (03) : 498 - 513
  • [42] A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
    Cesana, Marcella
    Cacchiarelli, Davide
    Legnini, Ivano
    Santini, Tiziana
    Sthandier, Olga
    Chinappi, Mauro
    Tramontano, Anna
    Bozzoni, Irene
    CELL, 2011, 147 (02) : 358 - 369
  • [43] Amelioration of insulin resistance using the additive effect of ferulic acid and resveratrol on vesicle trafficking for skeletal muscle glucose metabolism
    Kang, Bean-Bu
    Chiang, Been-Huang
    PHYTOTHERAPY RESEARCH, 2020, 34 (04) : 808 - 816
  • [44] Resveratrol improves insulin resistance of catch-up growth by increasing mitochondrial complexes and antioxidant function in skeletal muscle
    Zheng, Juan
    Chen, Lu-Lu
    Zhang, Hao-Hao
    Hu, Xiang
    Kong, Wen
    Hu, Di
    METABOLISM-CLINICAL AND EXPERIMENTAL, 2012, 61 (07): : 954 - 965
  • [45] Resveratrol Alleviates Lipid-Induced Insulin Resistance in Lean, But Not Severely Obese, Myotubes
    Turner, Kristen
    Park, Sanghee
    Beasley, Taylor
    Zheng, Donghai
    Houmard, Joseph
    FASEB JOURNAL, 2015, 29
  • [46] The functional role of long noncoding RNA in resistance to anticancer treatment
    Qu, Yidi
    Tan, Hor-Yue
    Chan, Yau-Tuen
    Jiang, Hongbo
    Wang, Ning
    Wang, Di
    THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY, 2020, 12
  • [47] RECENT ADVANCES IN THE MECHANISMS OF INSULIN RESISTANCE IN SKELETAL MUSCLE
    Vidal, H.
    OSTEOPOROSIS INTERNATIONAL, 2012, 23 : S399 - S400
  • [48] Effect of resistance exercise on insulin sensitivity of skeletal muscle
    Bo Wang
    Xu Luo
    Rong-Rong Li
    Ya-Na Li
    Yu-Chi Zhao
    World Journal of Meta-Analysis, 2021, 9 (02) : 101 - 107
  • [49] Role of the Wnt/catenin in resistance to insulin in skeletal muscle
    Christodoulou-Vafeiadou, E.
    Favier, M.
    Naviere, A.
    Maire, P.
    Guillet-Deniau, I.
    DIABETES & METABOLISM, 2011, 37 : A29 - A29
  • [50] Downregulation of serum long noncoding RNA GAS5 may contribute to insulin resistance in PCOS patients
    Lin, Haiyan
    Xing, Weijie
    Li, Yu
    Xie, Yanxin
    Tang, Xiaoshi
    Zhang, Qingxue
    GYNECOLOGICAL ENDOCRINOLOGY, 2018, 34 (09) : 784 - 788