Resveratrol Alleviates Skeletal Muscle Insulin Resistance by Downregulating Long Noncoding RNA

被引:12
|
作者
Liu, Zhihong [1 ,2 ,3 ]
Zhang, Zhimei [1 ,2 ]
Song, Guangyao [1 ,2 ]
Wang, Xing [4 ]
Xing, Hanying [4 ]
Wang, Chao [4 ]
机构
[1] Hebei Med Univ, Dept Internal Med, Shijiazhuang 050017, Hebei, Peoples R China
[2] Hebei Gen Hosp, Dept Endocrinol, Shijiazhuang 050051, Hebei, Peoples R China
[3] Hebei Med Univ, Dept Endocrinol, Second Hosp, Shijiazhuang 050000, Hebei, Peoples R China
[4] Hebei Gen Hosp, Hebei Key Lab Metab Dis, Shijiazhuang 050051, Hebei, Peoples R China
关键词
PATHWAY; CELLS; PROLIFERATION; MECHANISM; INVASION; OBESITY; SOCS-1; AXIS;
D O I
10.1155/2022/2539519
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Long noncoding RNA (lncRNA) is a crucial factor in the progression of insulin resistance (IR). Resveratrol (RSV) exhibits promising therapeutic potential for IR. However, there are few studies on whether RSV improves IR through lncRNA. This study aimed to determine whether RSV could influence the expression of lncRNA and to elucidate the underlying mechanism. Mice were divided into three groups: control group, high-fat diet (HFD) group, and HFD + RSV group. We conducted a high-throughput sequencing analysis to detect lncRNA and mRNA expression signatures and the ceRNA-network in the skeletal muscles of mice that were fed an HFD to induce IR. Hierarchical clustering, gene enrichment, and gene ceRNA-network analyses were subsequently conducted. Differentially expressed lncRNAs were selected and validated via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The biological functions of the selected lncRNAs were investigated by silencing the target genes via lentivirus transfection of C2C12 mouse myotube cells. RSV treatment reversed the expression of 338 mRNAs and 629 lncRNAs in the skeletal muscles of mice with HFD-induced IR. The results of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes database analyses indicated that the differentially expressed mRNAs modulated type II diabetes mellitus. After validating randomly selected lncRNAs via RT-qPCR, we identified a novel lncRNA, NONMMUT044897.2, which was upregulated in the HFD group and reversed with RSV treatment. Additionally, NONMMUT044897.2 was proven to function as a ceRNA of microRNA- (miR-) 7051-5p. Suppressor of Cytokine Signaling 1 (SOCS1) was confirmed as a target of miR-7051-5p. We further performed lentivirus transfection to knock down NONMMUT044897.2 in vitro and found that NONMMUT044897.2 silenced SOCS1 and potentiated the insulin signaling pathway. Hence, RSV mimicked the silencing effect of lentivirus transfection on NONMMUT044897.2. Our study revealed that RSV reduced IR in mouse skeletal muscles via the regulation of NONMMUT044897.2.
引用
收藏
页数:16
相关论文
共 50 条
  • [21] Aetiology and mechanisms of skeletal muscle insulin resistance
    Moro, C.
    CORRESPONDANCES EN METABOLISMES HORMONES DIABETES ET NUTRITION, 2024, 28 (04):
  • [22] Microvascular involvement in insulin resistance of skeletal muscle
    Clark, MG
    Barrett, EJ
    Richards, SM
    Clark, ADH
    Rattigan, S
    7TH WORLD CONGRESS FOR MICROCIRCULATION, 2001, : 31 - 37
  • [23] Insulin resistance and mitochondrial function in skeletal muscle
    Dela, Flemming
    Helge, Jorn Wulff
    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2013, 45 (01): : 11 - 15
  • [24] Insulin Resistance in Skeletal Muscle of Chronic Stroke
    Ryan, Alice S.
    Ortmeyer, Heidi
    Ivey, Frederick
    Hafer-Macko, Charlene
    STROKE, 2015, 46
  • [25] Skeletal muscle inflammation and insulin resistance in obesity
    Wu, Huaizhu
    Ballantyne, Christie M.
    JOURNAL OF CLINICAL INVESTIGATION, 2017, 127 (01): : 43 - 54
  • [26] Skeletal muscle insulin resistance: Fat or furnace?
    Goodpaster, HK
    OBESITY RESEARCH, 2001, 9 : 83S - 83S
  • [27] INSULIN RESISTANCE OF SKELETAL-MUSCLE IN OBESITY
    EVANS, DJ
    KALKHOFF, R
    KISSEBAH, AH
    INTERNATIONAL JOURNAL OF OBESITY, 1983, 7 (04) : 381 - 381
  • [28] Skeletal Muscle Insulin Resistance in Endocrine Disease
    Peppa, Melpomeni
    Koliaki, Chrysi
    Nikolopoulos, Panagiotis
    Raptis, Sotirios A.
    JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2010,
  • [30] Cellular mechanism of insulin resistance in skeletal muscle
    Petersen, KF
    Shulman, GI
    JOURNAL OF THE ROYAL SOCIETY OF MEDICINE, 2002, 95 : 8 - 13