Alteration of time-resolved autofluorescence properties of rat aorta, induced by diabetes mellitus

被引：1

作者：

Uherek, M. ^{[1
,2
]}

Ulicna, O. ^{[3
]}

Vancova, O. ^{[3
]}

Muchova, J. ^{[4
]}

Durackova, Z. ^{[4
]}

Sikurova, L. ^{[2
]}

Chorvat, D. ^{[1
,2
]}

机构：

[1] Ctr Int Laser, Dept Biophoton, Ilkovicova 3, Bratislava 84104, Slovakia

[2] Comenius Univ, Fac Math Phys & Informat, Dept Nucl Phys & Biophys, Mlynska Dolina F1, Bratislava 84248, Slovakia

[3] Comenius Univ, Fac Med, Dept Internal Med 3, Pharmacobiochem Lab, Spitalska 24, Bratislava 81372, Slovakia

[4] Comenius Univ, Fac Med, Inst Med Chem Biochem & Clin Biochem, Spitalska 24, Bratislava 81372, Slovakia

来源：

LASER PHYSICS | 2016年 / 26卷 / 10期

关键词：

diabetes mellitus; autofluorescence; time-resolved; fluorescence; laser-induced; spectroscopy; FLUORESCENCE LIFETIME; ENDOGENOUS FLUORESCENCE; MICROSCOPY; GLUCOSE; SPECTROSCOPY; INSULIN; MODEL; BARK; NADH; WALL;

D O I：

10.1088/1054-660X/26/10/105606

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Changes in autofluorescence properties of isolated rat aorta, induced by diabetes mellitus, were detected using time-resolved fluorescence spectroscopy with pulsed ultraviolet (UV) laser excitation. We demonstrated that time-resolved spectroscopy was able to detect changes in aorta tissues related to diabetes and unambiguously discriminate diabetic (tau(1) 0.63 +/- 0.05 ns, tau(2) 3.66 +/- 0.10 ns) samples from the control (tau(1) 0.76 +/- 0.03 ns, tau(2) 4.48 +/- 0.15 ns) group. We also report changes in the ratio of relative amplitudes of the two lifetime component in aorta tissue during diabetes, most likely related to the pseudohypoxic state with altered NADH homeostasis.

引用

页数：6

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