Alteration of time-resolved autofluorescence properties of rat aorta, induced by diabetes mellitus

被引：1
|
作者：
Uherek, M. [1 ,2 ]
Ulicna, O. [3 ]
Vancova, O. [3 ]
Muchova, J. [4 ]
Durackova, Z. [4 ]
Sikurova, L. [2 ]
Chorvat, D. [1 ,2 ]
机构：
[1] Ctr Int Laser, Dept Biophoton, Ilkovicova 3, Bratislava 84104, Slovakia
[2] Comenius Univ, Fac Math Phys & Informat, Dept Nucl Phys & Biophys, Mlynska Dolina F1, Bratislava 84248, Slovakia
[3] Comenius Univ, Fac Med, Dept Internal Med 3, Pharmacobiochem Lab, Spitalska 24, Bratislava 81372, Slovakia
[4] Comenius Univ, Fac Med, Inst Med Chem Biochem & Clin Biochem, Spitalska 24, Bratislava 81372, Slovakia
来源：
LASER PHYSICS | 2016年 / 26卷 / 10期
关键词：
diabetes mellitus; autofluorescence; time-resolved; fluorescence; laser-induced; spectroscopy; FLUORESCENCE LIFETIME; ENDOGENOUS FLUORESCENCE; MICROSCOPY; GLUCOSE; SPECTROSCOPY; INSULIN; MODEL; BARK; NADH; WALL;
D O I：
10.1088/1054-660X/26/10/105606
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Changes in autofluorescence properties of isolated rat aorta, induced by diabetes mellitus, were detected using time-resolved fluorescence spectroscopy with pulsed ultraviolet (UV) laser excitation. We demonstrated that time-resolved spectroscopy was able to detect changes in aorta tissues related to diabetes and unambiguously discriminate diabetic (tau(1) 0.63 +/- 0.05 ns, tau(2) 3.66 +/- 0.10 ns) samples from the control (tau(1) 0.76 +/- 0.03 ns, tau(2) 4.48 +/- 0.15 ns) group. We also report changes in the ratio of relative amplitudes of the two lifetime component in aorta tissue during diabetes, most likely related to the pseudohypoxic state with altered NADH homeostasis.
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页数：6
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