Effects of a Non-Thermal Atmospheric Pressure Plasma Jet with Different Gas Sources and Modes of Treatment on the Fate of Human Mesenchymal Stem Cells

被引:2
|
作者
Kang, Tae-Yun [1 ,2 ]
Kwon, Jae-Sung [1 ,2 ]
Kumar, Naresh [3 ]
Choi, Eun Ha [4 ]
Kim, Kwang-Mahn [1 ,2 ]
机构
[1] Yonsei Univ, Dept & Res Inst Dent Biomat & Bioengn, Coll Dent, Seoul 03722, South Korea
[2] Yonsei Univ, BK21 Plus Project, Coll Dent, Seoul 03722, South Korea
[3] Univ Antwerp, Dept Chem, Res Grp PLASMANT, B-2610 Wilrijk Antwerp, Belgium
[4] Kwangwoon Univ, Plasma Biosci Res Ctr, Seoul 01897, South Korea
来源
APPLIED SCIENCES-BASEL | 2019年 / 9卷 / 22期
关键词
non-thermal atmospheric pressure plasma; mesenchymal stem cells; tissue engineering; regenerative medicine; CHONDROGENIC DIFFERENTIATION; CYTOTOXICITY EVALUATION; BONE-MARROW; EXPRESSION;
D O I
10.3390/app9224819
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Despite numerous attempts to use human mesenchymal stem cells (hMSCs) in the field of tissue engineering, the control of their differentiation remains challenging. Here, we investigated possible applications of a non-thermal atmospheric pressure plasma jet (NTAPPJ) to control the differentiation of hMSCs. An air- or nitrogen-based NTAPPJ was applied to hMSCs in culture media, either directly or by media treatment in which the cells were plated after the medium was exposed to the NTAPPJ. The durations of exposure were 1, 2, and 4 min, and the control was not exposed to the NTAPPJ. The initial attachment of the cells was assessed by a water-soluble tetrazolium assay, and the gene expression in the cells was assessed through reverse-transcription polymerase chain reaction and immunofluorescence staining. The results showed that the gene expression in the hMSCs was generally increased by the NTAPPJ exposure, but the enhancement was dependent on the conditions of the exposure, such as the source of the gas and the treatment method used. These results were attributed to the chemicals in the extracellular environment and the reactive oxygen species generated by the plasma. Hence, it was concluded that by applying the best conditions for the NTAPPJ exposure of hMSCs, the control of hMSC differentiation was possible, and therefore, exposure to an NTAPPJ is a promising method for tissue engineering.
引用
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页数:10
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