Effects of a Non-Thermal Atmospheric Pressure Plasma Jet with Different Gas Sources and Modes of Treatment on the Fate of Human Mesenchymal Stem Cells

被引:2
|
作者
Kang, Tae-Yun [1 ,2 ]
Kwon, Jae-Sung [1 ,2 ]
Kumar, Naresh [3 ]
Choi, Eun Ha [4 ]
Kim, Kwang-Mahn [1 ,2 ]
机构
[1] Yonsei Univ, Dept & Res Inst Dent Biomat & Bioengn, Coll Dent, Seoul 03722, South Korea
[2] Yonsei Univ, BK21 Plus Project, Coll Dent, Seoul 03722, South Korea
[3] Univ Antwerp, Dept Chem, Res Grp PLASMANT, B-2610 Wilrijk Antwerp, Belgium
[4] Kwangwoon Univ, Plasma Biosci Res Ctr, Seoul 01897, South Korea
来源
APPLIED SCIENCES-BASEL | 2019年 / 9卷 / 22期
关键词
non-thermal atmospheric pressure plasma; mesenchymal stem cells; tissue engineering; regenerative medicine; CHONDROGENIC DIFFERENTIATION; CYTOTOXICITY EVALUATION; BONE-MARROW; EXPRESSION;
D O I
10.3390/app9224819
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Despite numerous attempts to use human mesenchymal stem cells (hMSCs) in the field of tissue engineering, the control of their differentiation remains challenging. Here, we investigated possible applications of a non-thermal atmospheric pressure plasma jet (NTAPPJ) to control the differentiation of hMSCs. An air- or nitrogen-based NTAPPJ was applied to hMSCs in culture media, either directly or by media treatment in which the cells were plated after the medium was exposed to the NTAPPJ. The durations of exposure were 1, 2, and 4 min, and the control was not exposed to the NTAPPJ. The initial attachment of the cells was assessed by a water-soluble tetrazolium assay, and the gene expression in the cells was assessed through reverse-transcription polymerase chain reaction and immunofluorescence staining. The results showed that the gene expression in the hMSCs was generally increased by the NTAPPJ exposure, but the enhancement was dependent on the conditions of the exposure, such as the source of the gas and the treatment method used. These results were attributed to the chemicals in the extracellular environment and the reactive oxygen species generated by the plasma. Hence, it was concluded that by applying the best conditions for the NTAPPJ exposure of hMSCs, the control of hMSC differentiation was possible, and therefore, exposure to an NTAPPJ is a promising method for tissue engineering.
引用
收藏
页数:10
相关论文
共 50 条
  • [31] Liquid assisted plasma enhanced chemical vapour deposition with a non-thermal plasma jet at atmospheric pressure
    Schaefer, Jan
    Fricke, Katja
    Mika, Filip
    Pokorna, Zuzana
    Zajickova, Lenka
    Foest, Ruediger
    THIN SOLID FILMS, 2017, 630 : 71 - 78
  • [32] Comparison of Direct and Indirect Non-Thermal Atmospheric Plasma Effect on Human Periodontal Ligament Stem Cells
    Jha, Nayansi
    Ryu, Jae Jun
    Choi, Eun Ha
    Han, Ihn
    JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, 2017, 7 (10) : 969 - 977
  • [33] On the Effect of Non-Thermal Atmospheric Pressure Plasma Treatment on the Properties of PET Film
    Maliszewska, Irena
    Gazinska, Malgorzata
    Lojkowski, Maciej
    Choinska, Emilia
    Nowinski, Daria
    Czapka, Tomasz
    Swieszkowski, Wojciech
    POLYMERS, 2023, 15 (21)
  • [34] Toxicity of Direct Non-Thermal Atmospheric Pressure Plasma Treatment of Living Tissue
    Kalghatgi, S.
    Dobrynin, D.
    Wu, A.
    Sensenig, R.
    Cerchar, E.
    Fridman, G.
    Balasubramanian, M.
    Fridman, A.
    Brooks, A.
    Barbee, K.
    Friedman, G.
    2009 IEEE PULSED POWER CONFERENCE, VOLS 1 AND 2, 2009, : 1121 - 1125
  • [35] Application of a Non-thermal Atmospheric Pressure Plasma Jet to the Decomposition of Salicylic Acid to Inorganic Carbon
    Kuroda, Kosuke
    Ishijima, Tatsuo
    Kaga, Toshiki
    Shiomomura, Kai
    Ninomiya, Kazuaki
    Takahashi, Kenji
    CHEMISTRY LETTERS, 2015, 44 (11) : 1473 - 1475
  • [36] Influence of Non-Thermal Atmospheric Pressure Plasma Jet on Extracellular Activity of α-Amylase in Aspergillus oryzae
    Veerana, Mayura
    Choi, Eun Ha
    Park, Gyungsoon
    APPLIED SCIENCES-BASEL, 2021, 11 (02): : 1 - 14
  • [37] On a non-thermal atmospheric pressure plasma jet used for the deposition of silicon-organic films
    Schaefer, Jan
    Sigeneger, Florian
    Foest, Ruediger
    Loffhagen, Detlef
    Weltmann, Klaus-Dieter
    EUROPEAN PHYSICAL JOURNAL D, 2018, 72 (05):
  • [38] TMAO and sorbitol attenuate the deleterious action of atmospheric pressure non-thermal jet plasma on α-chymotrypsin
    Attri, Pankaj
    Venkatesu, Pannuru
    Kaushik, Nagendra
    Choi, Eun Ha
    RSC ADVANCES, 2012, 2 (18) : 7146 - 7155
  • [39] Investigation of a non-thermal atmospheric pressure plasma jet in contact with liquids using fast imaging
    Adress, Wameedh
    Graham, Bill
    PLASMA SOURCES SCIENCE & TECHNOLOGY, 2021, 30 (09):
  • [40] Effects of Atmospheric-pressure Non-thermal Plasma Jets on Enzyme Solutions
    Attri, Pankaj
    Venkatesu, Pannuru
    Kaushik, Nagendra
    Han, Yong Gyu
    Nam, Chul Joo
    Choi, Eun Ha
    Kim, Key Sun
    JOURNAL OF THE KOREAN PHYSICAL SOCIETY, 2012, 60 (06) : 959 - 964