Evaluation of Potential Reference Genes for Relative Quantification by RT-qPCR in Different Porcine Tissues Derived from Feeding Studies

被引：30
|
作者：
Li, Qimeng [1 ]
Domig, Konrad Johann [2 ]
Ettle, Thomas [1 ]
Windisch, Wilhelm [3 ]
Mair, Christiane [1 ]
Schedle, Karl [1 ]
机构：
[1] Univ Nat Resources & Life Sci, Inst Anim Nutr Prod & Nutr Physiol APN, A-1190 Vienna, Austria
[2] Univ Nat Resources & Life Sci, Inst Food Sci, Dept Food Sci & Technol, A-1190 Vienna, Austria
[3] Tech Univ Muenchen Weihenstephan, Chair Anim Nutr, D-85350 Freising Weihenstephan, Germany
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2011年 / 12卷 / 03期
关键词：
sus scrofa; RT-qPCR; reference genes; gene stability; REVERSE TRANSCRIPTION-PCR; POLYMERASE CHAIN-REACTION; MESSENGER-RNA EXPRESSION; HOUSEKEEPING GENES; NORMALIZATION; GROWTH; CHOLECYSTOKININ; SELECTION; PATTERN; BLOOD;
D O I：
10.3390/ijms12031727
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Five potential reference genes for RT-qPCR application, namely histone H3, beta-actin, GAPDH, ubiquitin and 18S rRNA, were evaluated for normalization of gene expression in four selected tissues ( liver, kidney, thyroid and abdominal fat). Tissues were derived from fattening pigs exposed to different amounts and type of dietary iodine. Two software applications (geNorm and NormFinder) were used to evaluate the stability of the potential reference genes. All studied genes displayed high expression stability but different stability patterns between the investigated tissues. The results suggest GAPDH and 18S rRNA as reference genes applicable in all tissues investigated. Beta-actin and histone H3 are suitable reference genes for all tissues investigated except fat. In contrast, ubiquitin should be excluded from use as a reference gene in the porcine tissues analyzed due to variations in expression levels, despite the good expression stability.
引用
收藏
页码：1727 / 1734
页数：8
相关论文
共 50 条
  • [31] Selection and validation of reference genes for RT-qPCR normalization in different tissues of milk thistle (Silybum marianum, Gaert.)
    Fulvio, Flavia
    Martinelli, Tommaso
    Paris, Roberta
    GENE, 2021, 768
  • [32] Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions
    Zhang, Chen
    Li, Tong
    Hou, Cheng-Lin
    Shen, Xiao-Ye
    AMB EXPRESS, 2017, 7
  • [33] Selection of potential reference genes for RT-qPCR in the plant pathogenic fungus Colletotrichum fructicola
    Chen, Xingzhou
    Chen, Xinggang
    Tan, Qian
    He, Yuan
    Wang, Zhikai
    Zhou, Guoying
    Liu, Junang
    FRONTIERS IN MICROBIOLOGY, 2022, 13
  • [34] RT-qPCR normalization of reference genes in different lifehistory stages of Gracilaria vermiculophylla(Rhodophyta)
    Yingyue ZHANG
    Jinxin YANG
    Ze YANG
    Cong QI
    Di XU
    JournalofOceanologyandLimnology, 2023, 41 (05) : 1910 - 1917
  • [35] Validation of reference genes for RT-qPCR studies in Stevia rebaudiana in response to elicitor agents
    Lucho, Simone Ribeiro
    do Amaral, Marcelo Nogueira
    Benitez, Leticia Carvalho
    Milech, Cristini
    Kleinowski, Alitcia Moraes
    Bianchi, Valmor Joao
    Bolacel Braga, Eugenia Jacira
    PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2018, 24 (05) : 767 - 779
  • [36] Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions
    Chen Zhang
    Tong Li
    Cheng-Lin Hou
    Xiao-Ye Shen
    AMB Express, 7
  • [37] Validation of reference genes for RT-qPCR studies in Stevia rebaudiana in response to elicitor agents
    Simone Ribeiro Lucho
    Marcelo Nogueira do Amaral
    Letícia Carvalho Benitez
    Cristini Milech
    Alitcia Moraes Kleinowski
    Valmor João Bianchi
    Eugenia Jacira Bolacel Braga
    Physiology and Molecular Biology of Plants, 2018, 24 : 767 - 779
  • [38] Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions
    Chen, Lei
    Zhong, Hai-ying
    Kuang, Jian-fei
    Li, Jian-guo
    Lu, Wang-jin
    Chen, Jian-ye
    PLANTA, 2011, 234 (02) : 377 - 390
  • [39] Validation of reference genes for cardiac RT-qPCR studies spanning the fetal to adult period
    Bose, Karthikeyan
    Louey, Samantha
    Jonker, Sonnet S.
    METHODSX, 2024, 13
  • [40] Selection of reference genes for RT-qPCR studies in blood of beluga whales (Delphinapterus leucas)
    Chen, I-Hua
    Wang, Jiann-Hsiung
    Chou, Shih-Jen
    Wu, Yeong-Huey
    Li, Tsung-Hsien
    Leu, Ming-Yih
    Chang, Wen-Been
    Yang, Wei Cheng
    PEERJ, 2016, 4
12345