Evaluation of Potential Reference Genes for Relative Quantification by RT-qPCR in Different Porcine Tissues Derived from Feeding Studies

被引：30
|
作者：
Li, Qimeng [1 ]
Domig, Konrad Johann [2 ]
Ettle, Thomas [1 ]
Windisch, Wilhelm [3 ]
Mair, Christiane [1 ]
Schedle, Karl [1 ]
机构：
[1] Univ Nat Resources & Life Sci, Inst Anim Nutr Prod & Nutr Physiol APN, A-1190 Vienna, Austria
[2] Univ Nat Resources & Life Sci, Inst Food Sci, Dept Food Sci & Technol, A-1190 Vienna, Austria
[3] Tech Univ Muenchen Weihenstephan, Chair Anim Nutr, D-85350 Freising Weihenstephan, Germany
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2011年 / 12卷 / 03期
关键词：
sus scrofa; RT-qPCR; reference genes; gene stability; REVERSE TRANSCRIPTION-PCR; POLYMERASE CHAIN-REACTION; MESSENGER-RNA EXPRESSION; HOUSEKEEPING GENES; NORMALIZATION; GROWTH; CHOLECYSTOKININ; SELECTION; PATTERN; BLOOD;
D O I：
10.3390/ijms12031727
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Five potential reference genes for RT-qPCR application, namely histone H3, beta-actin, GAPDH, ubiquitin and 18S rRNA, were evaluated for normalization of gene expression in four selected tissues ( liver, kidney, thyroid and abdominal fat). Tissues were derived from fattening pigs exposed to different amounts and type of dietary iodine. Two software applications (geNorm and NormFinder) were used to evaluate the stability of the potential reference genes. All studied genes displayed high expression stability but different stability patterns between the investigated tissues. The results suggest GAPDH and 18S rRNA as reference genes applicable in all tissues investigated. Beta-actin and histone H3 are suitable reference genes for all tissues investigated except fat. In contrast, ubiquitin should be excluded from use as a reference gene in the porcine tissues analyzed due to variations in expression levels, despite the good expression stability.
引用
收藏
页码：1727 / 1734
页数：8
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