Selenium-containing compounds in baker's yeast (Saccharomyces cerevisiae)

被引：0

作者：

Kyriakopoulos, A ^{[1
]}

Röthlein, D ^{[1
]}

Pfeifer, H ^{[1
]}

Gessner, H ^{[1
]}

Behne, D ^{[1
]}

机构：

[1] Hahn Meitner Inst Kernforsch Berlin GmbH, Dept Trace Elements Hlth & Nutr, D-14109 Berlin, Germany

来源：

MICROELEMENTS AND TRACE ELEMENTS | 1998年

关键词：

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The distribution of selenium in subcellular fractions of Saccharomyces cerevisiae was investigated after cultivation of the yeast cells in a medium with added Se-75-selenite. The major part of the tracer taken up by the cells was contained in loosely bound small compounds with molecular masses below 1 kDa, present on the cell walls and the outer membranes of the mitochondrial and microsomal fractions. After separation of the proteins in the subcellular fractions by means of SDS-PAGE a Se-75-containing cytosolic protein band with the molecular mass of 25 kDa was found, which migrated with the same velocity as the GSH-Px subunit isolated from rat liver. As the yeast cytosol showed enzymatic glutathione peroxidase activity, the results might suggest that the selenium-containing 25 kDa-protein in Saccharomyces cerevisiae is similar to the GSH-Px subunit.

引用

页码：740 / 745

页数：6

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