Selenium-containing compounds in baker's yeast (Saccharomyces cerevisiae)

被引:0
|
作者
Kyriakopoulos, A [1 ]
Röthlein, D [1 ]
Pfeifer, H [1 ]
Gessner, H [1 ]
Behne, D [1 ]
机构
[1] Hahn Meitner Inst Kernforsch Berlin GmbH, Dept Trace Elements Hlth & Nutr, D-14109 Berlin, Germany
关键词
D O I
暂无
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The distribution of selenium in subcellular fractions of Saccharomyces cerevisiae was investigated after cultivation of the yeast cells in a medium with added Se-75-selenite. The major part of the tracer taken up by the cells was contained in loosely bound small compounds with molecular masses below 1 kDa, present on the cell walls and the outer membranes of the mitochondrial and microsomal fractions. After separation of the proteins in the subcellular fractions by means of SDS-PAGE a Se-75-containing cytosolic protein band with the molecular mass of 25 kDa was found, which migrated with the same velocity as the GSH-Px subunit isolated from rat liver. As the yeast cytosol showed enzymatic glutathione peroxidase activity, the results might suggest that the selenium-containing 25 kDa-protein in Saccharomyces cerevisiae is similar to the GSH-Px subunit.
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收藏
页码:740 / 745
页数:6
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