Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts

被引：40
|
作者：
Yamayoshi, Seiya [1 ]
Fukuyama, Satoshi [1 ,2 ]
Yamada, Shinya [1 ]
Zhao, Dongming [1 ,2 ]
Murakami, Shin [3 ]
Uraki, Ryuta [1 ]
Watanabe, Tokiko [1 ,2 ]
Tomita, Yuriko [1 ,2 ]
Neumann, Gabriele [4 ]
Kawaoka, Yoshihiro [1 ,2 ,3 ,4 ]
机构：
[1] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Virol, Tokyo, Japan
[2] Japan Sci & Technol Agcy, ERATO Infect Induced Host Responses Project, Saitama, Japan
[3] Univ Tokyo, Inst Med Sci, Int Res Ctr Infect Dis,Dept Special Pathogens, Minato Ku, Tokyo, Japan
[4] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
基金：
日本科学技术振兴机构;
关键词：
HUMAN INFECTIONS; MOLECULAR-BASIS; TRANSMISSION; FERRETS; REPLICATION; GENE;
D O I：
10.1038/srep08039
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells, and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses.
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页数：4
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