Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts

被引：40

作者：

Yamayoshi, Seiya ^{[1
]}

Fukuyama, Satoshi ^{[1
,2
]}

Yamada, Shinya ^{[1
]}

Zhao, Dongming ^{[1
,2
]}

Murakami, Shin ^{[3
]}

Uraki, Ryuta ^{[1
]}

Watanabe, Tokiko ^{[1
,2
]}

Tomita, Yuriko ^{[1
,2
]}

Neumann, Gabriele ^{[4
]}

Kawaoka, Yoshihiro ^{[1
,2
,3
,4
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Virol, Tokyo, Japan

[2] Japan Sci & Technol Agcy, ERATO Infect Induced Host Responses Project, Saitama, Japan

[3] Univ Tokyo, Inst Med Sci, Int Res Ctr Infect Dis,Dept Special Pathogens, Minato Ku, Tokyo, Japan

[4] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

日本科学技术振兴机构;

关键词：

HUMAN INFECTIONS; MOLECULAR-BASIS; TRANSMISSION; FERRETS; REPLICATION; GENE;

D O I：

10.1038/srep08039

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells, and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses.

引用

页数：4

共 50 条

[21] The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens
Hao, Xiaoli
Wang, Xiaoquan
Hu, Jiao
Gu, Min
Wang, Jiongjiong
Deng, Yonghuan
Jiang, Daxiu
He, Dongchang
Xu, Haixu
Yang, Yi
Hu, Zenglei
Chen, Sujuan
Hua, Shunlin
Liu, Xiaowen
Shang, Shaobin
Peng, Daxin
Jiao, Xinan
Liu, Xiufan
VIROLOGY, 2019, 535 : 218 - 226
[22] Quantifying the Fitness Advantage of Polymerase Substitutions in Influenza A/H7N9 Viruses during Adaptation to Humans
Fonville, Judith M.
Burke, David F.
Lewis, Nicola S.
Katzelnick, Leah C.
Russell, Colin A.
PLOS ONE, 2013, 8 (09):
[23] Mammalian Pathogenesis and Transmission of Avian Influenza A(H7N9) Viruses, Tennessee, USA, 2017
Belser, Jessica A.
Brock, Nicole
Sun, Xiangjie
Jones, Joyce
Zanders, Natosha
Hodges, Erin
Pulit-Penaloza, Joanna A.
Wentworth, David
Tumpey, Terrence M.
Davis, Todd
Maines, Taronna R.
EMERGING INFECTIOUS DISEASES, 2018, 24 (01) : 149 - 152
[24] Risk Assessment of Fifth-Wave H7N9 Influenza A Viruses in Mammalian Models
Sun, Xiangjie
Belser, Jessica A.
Pappas, Claudia
Pulit-Penaloza, Joanna A.
Brock, Nicole
Zeng, Hui
Creager, Hannah M.
Le, Shoshona
Wilson, Malania
Lewis, Amanda
Stark, Thomas J.
Shieh, Wun-Ju
Barnes, John
Tumpey, Terrence M.
Maines, Taronna R.
JOURNAL OF VIROLOGY, 2019, 93 (01)
[25] Codon usage characteristics of PB2 gene in influenza A H7N9 virus from different host species
Li Gun
Pan Haixian
Ren Yumiao
Tian Han
Lu Jingqi
Zhang Liguang
INFECTION GENETICS AND EVOLUTION, 2018, 65 : 430 - 435
[26] The first H7N9 influenza case of Shanghai in 2014: Reemerged virus with different origin of PB2 gene
He, Yanchao
Zhang, Wanju
Xu, L.
Mei, Zhoufang
Qian, Ling
Hu, Yunwen
Jie, Zhijun
EUROPEAN RESPIRATORY JOURNAL, 2014, 44
[27] Emergence and development of H7N9 influenza viruses in China
Zhu, Huachen
Lam, Tommy Tsan-Yuk
Smith, David Keith
Guan, Yi
CURRENT OPINION IN VIROLOGY, 2016, 16 : 106 - 113
[28] Influenza H7N9 and H9N2 Viruses: Coexistence in Poultry Linked to Human H7N9 Infection and Genome Characteristics
Yu, Xinfen
Jin, Tao
Cui, Yujun
Pu, Xiaoying
Li, Jun
Xu, Jin
Liu, Guang
Jia, Huijue
Liu, Dan
Song, Shili
Yu, Yang
Xie, Li
Huang, Renjie
Ding, Hua
Kou, Yu
Zhou, Yinyan
Wang, Yayu
Xu, Xun
Yin, Ye
Wang, Jian
Guo, Chenyi
Yang, Xianwei
Hu, Liangping
Wu, Xiaopeng
Wang, Hailong
Liu, Jun
Zhao, Guoqiu
Zhou, Jiyong
Pan, Jingcao
Gao, George F.
Yang, Ruifu
Wang, Jun
JOURNAL OF VIROLOGY, 2014, 88 (06) : 3423 - 3431
[29] Pandemic potential of avian influenza A (H7N9) viruses
Watanabe, Tokiko
Watanabe, Shinji
Maher, Eileen A.
Neumann, Gabriele
Kawaoka, Yoshihiro
TRENDS IN MICROBIOLOGY, 2014, 22 (11) : 623 - 631
[30] Ostrich Involvement in the Selection of H5N1 Influenza Virus Possessing Mammalian-Type Amino Acids in the PB2 Protein
Shinya, Kyoko
Makino, Akiko
Ozawa, Makoto
Kim, Jin Hyun
Sakai-Tagawa, Yuko
Ito, Mutsumi
Quynh Mai Le
Kawaoka, Yoshihiro
JOURNAL OF VIROLOGY, 2009, 83 (24) : 13015 - 13018

← 1 2 3 4 5 →