Polymerase-chain-reaction-based detection of individual polyhydroxyalkanoate synthase phaC1 and phaC2 genes

被引：12
|
作者：
Solaiman, DKY [1 ]
机构：
[1] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 04期
关键词：
Comamonas; P; corrugata; PCR; PHA synthase; poly(hydroxyalkanoates); Pseudomonas oleovorans;
D O I：
10.1023/A:1014017609866
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A semi-nested PCR method for the specific and individual amplification of type II phaC1 and phaC2 subgenomic fragments containing the catalytically important lipase box-like sequence and the phosphopantetheine binding site was developed. Direct sequencing of these DeltaphaC1 and DeltaphaC2 fragments is possible, thus greatly facilitating the characterization of these genes. The method was used to show that three strains of Pseudomonas oleovorans harbor different pha loci, while five strains of P. corrugata contain identical DeltaphaC1 and DeltaphaC2 subgenomic fragments.
引用
收藏
页码：245 / 250
页数：6
相关论文
共 50 条
  • [41] Droplet Digital Polymerase Chain Reaction for Detection of Herpes Simplex Virus Type 1 and 2 in Clinical Samples
    Kim, H.
    Hur, M.
    Chung, H. -J.
    Moon, H. -W.
    Yun, Y. -M.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2019, 21 (03): : S38 - S38
  • [42] Comparison of different polymerase chain reaction methods for detection of herpes simplex virus types 1 and 2 encephalitis
    Altuglu I.
    Zeytinoglu A.
    Sirin H.
    Yuceyar N.
    Erensoy S.
    European Journal of Clinical Microbiology and Infectious Diseases, 2006, 25 (10): : 669 - 671
  • [43] Real-time reverse transcription polymerase chain reaction detection and quantification of t(1;19) (E2A-PBX1) fusion genes associated with leukaemia
    Curry, JD
    Glaser, MC
    Smith, MT
    BRITISH JOURNAL OF HAEMATOLOGY, 2001, 115 (04) : 826 - 830
  • [44] Identification of the novel allele HLA-DRB1*150205 by polymerase chain reaction-sequence-based typing in a Chinese individual
    Zhu, F. -M.
    Zhang, W.
    He, J.
    Yan, L. -X.
    TISSUE ANTIGENS, 2009, 74 (02): : 173 - 175
  • [45] Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction
    Chassagne, Laurent
    Pradel, Nathalle
    Robin, Frederic
    Livrelli, Valerie
    Bonnet, Richard
    Delmas, Julien
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2009, 64 (01) : 98 - 101
  • [46] Development and routine application of fast diagnostic technique based on polymerase chain reaction for the detection of equine-herpesvirus-2
    Szendröi, A
    Bakonyi, T
    Bagócs, A
    Ozvald, I
    Hornyák, A
    Rusvai, M
    MAGYAR ALLATORVOSOK LAPJA, 2005, 127 (10) : 579 - 585
  • [47] Parvovirus B19 genotypes 1 and 2 detection with real-time polymerase chain reaction assays
    Koppelman, M. H. G. M.
    Rood, I. G. H.
    Fryer, J. F.
    Baylis, S. A.
    Cuypers, H. T. M.
    VOX SANGUINIS, 2007, 93 (03) : 208 - 215
  • [48] Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis
    Ramos, Carlos A. N.
    Araujo, Flabio R.
    Souza, Ingrid I. F.
    Bacanelli, G.
    Luiz, Hera L.
    Russi, Livia S.
    Oliveira, Renato H. M.
    Soares, Cleber O.
    Rosinha, Gracia M. S.
    Alves, Leucio C.
    VETERINARY PARASITOLOGY, 2011, 176 (01) : 79 - 83
  • [49] Polymerase chain reaction-based temporal detection of Pasteurella multocida B:2 in the blood of experimentally infected mice
    Hazari, Ragini
    Kumar, Aman
    Maan, Sushila
    Kumar, Arvind
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 2018, 55 (04): : 286 - 289
  • [50] Validation of rapid polymerase chain reaction-based detection of all length polymorphisms in the UGT 1A1 gene promoter
    Huang, Chih-Kang
    Dulau, Alina
    Su-Rick, Chiao-Ju
    Pan, Qiulu
    DIAGNOSTIC MOLECULAR PATHOLOGY, 2007, 16 (01) : 50 - 53
12345